中国蔬菜Issue(10)：58-63,6.
魔芋浅层液体组织培养体系研究
Studied on Shallow Liquid Tissue Culture System in Amorphophallus konjac K.Koch
摘要
Abstract
Amorphophallus konjac K.Koch tissue culture was studied in liquid and solid MS medium supplemented with different hormone proportion.The result showed that shallow liquid medium was prior to solid medium in browning control, callus growth speed, number of differentiation buds, robustness of roots and low cost.MS+6-BA 0.5 mg · L-1+NAA 0.5 mg · L-1+Sucrose 3.0 ％+0.1％ PVP ( pH 5.8 ) could be used for primary culture.Alternative culture in MS+6-BA 2.0 mg · L-1+NAA 0.5 mg · L-1+Sucrose 3.0 ％+0.1％ PVP ( pH 5.8 ) and MS+6-BA 1.0 mg·L-1+NAA 0.5 mg·L-1+Sucrose 3.0 ％+0.1％ PVP ( pH 5.8 ) could produce tissue culture plantlets in batch and continuously.Rooting medium could be 1/2MS+IAA 0.5 mg · L-1+Suerose 1.5 ％+0.1％ PVP ( pH 5.8 ).3 types of ealli were found after subculture for 3 times.Remarkable difference existed in the A, B and C types in appearance, organization structure and capacities of re-differentiation.Calli of A and B-type were chosen for bud differentiation, both in test and application, which could produce a stable and efficient differentiation system.关键词
魔芋/浅层液体培养/褐变/愈伤组织Key words
Amorphophallus konjac K.Koch/ Shallow liquid tissue culture/ Browning/ Callus分类
农业科技引用本文复制引用
胡楠,刘海利,王启军,牛义,张盛林..魔芋浅层液体组织培养体系研究[J].中国蔬菜,2011,(10):58-63,6.基金项目
重庆市科委攻关项目(CSTC.2010AC1013),中央高校基本科研业务费专项资金资助项目(XDJK2009B022) （CSTC.2010AC1013）
