ISSR-PCR分子标记法鉴别宁杞1号与雄性不育枸杞OA
Identification of No. 1 and Male Sterility of Lycium barbarum L. by ISSR-PCR Molecular Marker Techniqe
[目的]探索在核酸分子水平上鉴别道地药材宁夏枸杞中不同的品种.[方法]从50务ISSR引物中筛选合适的引物对宁杞1号与雄性不育2个品种进行PCR扩增及电泳分析,寻找特征位点.[结果]1条ISSR引物扩增出较为明显的多态性特征条带,可用于宁杞1号与雄性不育的鉴别.[结论]ISSR-PCR作为一种简便、可靠的分子标记方法,可用于枸杞的鉴别.
[Objective]To identify genuine medicnal material of Lycium L.by ISSR-PCR molecular marker technique on the level of DNA molecular. [ Method ] Suitable primers were screened out from 50 ISSR primers (lnter-simple sequence repeat ) ,and then used in PCR amplification and electrophoretic analysis for distinguishging Lycium barbarum L from No. 1 and male sterility by finding specific sites. [ Result ] One ISSR primer could amplify polymorphic bands that were su…查看全部>>
思彬彬;王镇
北方民族大学生物科学与工程学院,宁夏银川,750021北方民族大学生物科学与工程学院,宁夏银川,750021
农业科技
枸杞(Lycium barbarum L.)ISSR鉴别
Lycium barbarum L. ISSR Identification
《安徽农业科学》 2011 (14)
8309-8310,2
北方民族大学资助项目(2007Y049).
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