安徽医科大学学报2011，Vol.46Issue(9)：870-874,5.
骨髓间充质干细胞移植联合高压氧治疗兔脊髓冲击伤
Therapeutic effects of bone marrow mesenchymal stem cells transplantation combined with hyperbaric oxygen on spinal cord blast injury
摘要
Abstract
Objective To therapy spinal cord injury by bone marrow mesenchymal stem cells and hyperbaric oxygen, to evaluate whether their combined treatment effect is better than their respective effect when used separately or not. Methods Established the models of T9-T10 spinal cord injuries, evaluated the extent of injuries by making kinematics score and somatosensory evoked potential score of each rabbit after 12 h , then 48 rabbits were randomly divided into four groups( n = 12 ) , which treated nothing, bone marrow mesenchymal stem cells transplantation , hyperbaric oxygen intervention,combined treatments .respectively. Six weeks later. evaluated with kinematics score,and immunofluorescence tracing method, toluidine blue stain, immunohistochemistry ( Bax, Bcl-2 detection of apoptosis ), and estimated the therapeutic efficacy of each group. Results After six weeks ,we found that the treatment of combined treatment on spinal cord injury was better than the other groups significantly( P ＜ 0. 05 ). Conclusion The therapeutic effects of bone marrow mesenchymal stem cells transplantation combined with hyperbaric oxygen on spinal cord blast injury is clear. Two therapy can play a synergistic effect and result in better treatment.关键词
骨髓/间质干细胞移植/高压氧/脊髓损伤/治疗Key words
bone marrow/mesenchymal stem cell transpltation/hyperbaric oxygenation/spinal cord injuries/therapy分类
医药卫生引用本文复制引用
裴少保,方健,王海峰,梅厚东,张迪,蒋传海..骨髓间充质干细胞移植联合高压氧治疗兔脊髓冲击伤[J].安徽医科大学学报,2011,46(9):870-874,5.基金项目
解放军105医院院管重点课题(编号:2010YG18) （编号:2010YG18）
