深圳大学学报（理工版）2011，Vol.28Issue(3)：237-242,6.
莱茵衣藻硫胁迫相关microRNA检测及其靶基因分析
MicroRNAs quantification and related target genes for response to sulfur deprivation in Chlamydomonas reinhardtii
摘要
Abstract
Two small RNA libraries, which responded to sulfur-replete and sulfur-deprivation condition, were gencrated by using stem-loop primers in Chlamydomonas reinhardtii. Three microRNAs ( miRNA1145.2, miRNA1146 and miRNA1158 ) were quantified by using SYBR(R) green reverse transcription polymerase chain reaction (RTPCR) detection with U4 snoRNA as internal reference gene. Their target genes were predicted by the Web MicroRNAs Designer (WMD3). The results showed that all three microRNAs expressions were up-regulated under sulfur deprivation in Chlamydomonas reinhardtii. The ratio of relative abundance with/without sulfur deprivation for miRNA1145.2, miRNA1146 and miRNA1158 were 3.11, 2.38 and 3.67, respectively. The miRNA1145.2 was able to target the thiolase gene to influence the fatty acid metabolism. The miRNA1146 targeted the genes for ubiquinone/menaquinone biosynthesis methyltransferase to control the ubiquinone metabolism. The miRNA1158 targeted the 6-phosphogluconate dehydrogenase genes to regulate the pentose phosphate pathway. Results show that the photosynthetic metabolism could be changed by the expression of endogenous miRNAs to regulate their target genes. And we can also design miRNAs to regulate any specific genes that catch our attention.关键词
生物工程/分子生物学/莱茵衣藻/缺硫胁迫/小分子核糖核酸/实时定量反转录聚合酶链式反应Key words
bioengineering/ molecular biology/ Chlamydomonas reinhardtii/ sulfur deprivation/ micro ribonucleic acid (microRNA)/ real-time RT-PCR分类
生物科学引用本文复制引用
胡章立,舒龙飞,苟德明..莱茵衣藻硫胁迫相关microRNA检测及其靶基因分析[J].深圳大学学报（理工版）,2011,28(3):237-242,6.基金项目
国家自然科学基金资助项目(31070323) （31070323）
