中国病理生理杂志2011，Vol.27Issue(7)：1393-1397,5.DOI:10.3969/j.issn.1000-4718.2011.07.026
不同浓度地塞米松体外对人成骨细胞和破骨细胞分化的影响
Effect of dexamethasone on differentiation of human osteoblast and osteoclast in vitro
摘要
Abstract
AIM: To investigate the effect of dexamethasone ( DEX ) on differentiation of human osteoblasts ( OBs ) and osteoclasts ( OCs ) in vitro.METHODS: The normal human bone marrow mesenchymal stem cells ( MSCs ) were stimulated with the inducer mainly containing DEX in vitro.Alizarin red staining and alkaline phosphatase ( ALP ) kit were used to identify the formation of calcium nodules among OBs and ALP levels in OBs, respectively.The levels of ALP were quantified again after the concentration of DEX was changed, and the expression of phosphorylated GSK - 3β in OBs was detected by Western blotting.Human bone marrow mononuclear cells ( BMMs ) were induced by exogenous receptor activator of nuclear factor kappa B ligand ( RANKL ) for 14 days.Tartrate - resistant acidic phosphatase staining was used to identity the formation of OCs, and the downstream signaling pathway of RANKL was detected by Western blotting.The level of soluble RANKL( sRANKL ) in the supernatants and the expression of RANKL in MSCs were measured by ELISA and Western blotting,respectively, when MSCs were stimulated with different concentrations of DEX for 7 days.RESULTS: Calcium nodules were formed when MSCs were stimulated with DEX at concentration of 0.1 μmol/L.The level of ALP and the protein expression of phospho - GSK - 3 β in OBs gradually increased by treating the cells with DEX at the concentrations within the range of 0.001 -0.1 μmol/L.However, the level of ALP and the protein expression of phospho - GSK -3β were decreased as the concentrations of DEX further increased to the range of 0.1 -10 μmol/L.BMMs were differeniated into OCs by exogenous RANKL, and the phosphorylations of IκBα, SPAK/JNK, p38 MAPK and p44/42 MAPK in BMMs increased.When MSCs were stimulated with DEX at the concentrations within 0.001 - 10 μmol/L, the level of sRANKL in the supernatants and the protein expression of RANKL in MSCs gradually increased, and the level of sRANKL was positively correlated with the concentration of DEX ( r =0.821 ,P ＜0.05 ).CONCLUSION: DEX at low concentrations promotes the differentiation of OBs and OCs.DEX at high concentrations only promotes the differentiation of OCs, not that of OBs.关键词
地塞米松/成骨细胞/破骨细胞Key words
Dexamethasone/ Osteoblasts/ Osteoclasts分类
医药卫生引用本文复制引用
许多荣,许辉茹,王荷花,李娟,吴洪福,邓宇斌..不同浓度地塞米松体外对人成骨细胞和破骨细胞分化的影响[J].中国病理生理杂志,2011,27(7):1393-1397,5.基金项目
国家自然科学基金资助项目(No.30670997) （No.30670997）
教育部归国人员启动基金资助项目(No.2005546) （No.2005546）
中山大学附属第一医院人才支持计划资助项目(No.18700108) （No.18700108）
