中国中西医结合杂志2011，Vol.31Issue(5)：680-683,4.
慢性心理应激对血管内皮功能障碍大鼠的影响及通心络的干预作用
Effect of Chronic Psychological Stress on Vascular Endothelial Dysfunction Rats and Intervention of Tongxinluo on it
摘要
Abstract
Objective To observe the effect of chronic psychological stress on vascular endothelial dysfunction rats and to explore the intervention and mechanism of Tongxinluo (TXL) on it.Methods Forty male Wistar rats were randomly divided into the normal control group (with no modeling), the endothelial dysfunction group (the HCY group), the psychological stress group (the model group), and TXL group, ten in each group.Rats in the latter three groups were fed with 3％ high methionine diet to duplicate vascular endothelial dysfunction (VED) model.In addition,chronic psychological stress was applied in VED rats using repeated binding method.TXL at the dose of 1.2 g/kg body weight was given by gastrogavage.The plasma endothelin (ET) and angiotensin Ⅱ (Ang Ⅱ), serum cortisone (CORT)were detected by radioimmunoassay.The serum nitric oxide (NO) was detected by nitrate reductase.Ultrastructural changes of aortic endothelial cells were observed by transmission electron microscope.Serum levels of norepinephrine (NE) and epinephrin (E) were detected by enzyme linked immunosorbent assay (ELISA).Results Compared with the plasma ET level and the serum NO level in the HCY group (161.70 ±13.96 pg/mL and 26.82 ±13.03 μmol/L), the plasma ET level obviously increased (178.25 ±21.85 pg/mL) ( P＜0.05) and the serum NO level decreased (24.91 ±9.95 μmol/L, P＞0.05), levels of CORT, NE, and E obviously increased in the model group (all P＜0.05).Ultrastructural changes of aortic endothelial cells were obviously injured.Compared with the model group, the plasma ET level (154.74±13.27 pg/mL), Ang Ⅱ, CORT, NE, and E obviously decreased (P＜0.05, P＜0.01), the serum NO level obviously increased (34.44 ±18.35 μmol/L, P＜0.05).Ultrastructural changes of aortic endothelial cells were obviously improved.Conclusions Chronic psychological stress could obviously aggravate endothelial injury in VED rats.TXL showed protection on the vascular endothelial structure and function.关键词
慢性束缚应激/血管内皮结构和功能/下丘脑-垂体-肾上腺皮质轴/肾素-血管紧张素系统/交感神经系统/通心络Key words
chronic psychological stress/ the vascular endothelial structure and function/ hypothalamicpituitary-adrenal axis/ renin-angiotensin system/ sympathetic nervous system/ Tongxinluo引用本文复制引用
吴相春,来静,吴相锋,贾振华,魏聪,袁国强,王宏涛,冯书文..慢性心理应激对血管内皮功能障碍大鼠的影响及通心络的干预作用[J].中国中西医结合杂志,2011,31(5):680-683,4.基金项目
973国家重点基础研究发展计划资助项目(No.2005CB523301) （No.2005CB523301）
