中国中西医结合杂志2011，Vol.31Issue(5)：690-695,6.
心肝宝胶囊对阿霉素肾病大鼠的肾脏保护作用
The Renal Protective Effect of Xinganbao Capsule in Adriamycin Induced Nephropathy Rats
摘要
Abstract
Objective To study the renal protective effect of Xinganbao Capsule on rats with adriamycin induced nephropathy (AIN). Methods Forty male SD rats were randomly divided into four groups, i.e. the normal control group (N), the AIN model group (M), the Benazepril group (B),end the Xinganbao Capsule group (X). AIN rat model was established by left unilateral nephrectomy and repeated caudal vein injection of adriamycin. Gastric perfusion of Xinganbao Capsule (at the dose of 500 mg/kg per day) and Benazepril (at the dose of 4 mg/kg per day) was given to rats in the X group and the B group respectively one week after nephrectomy. Rats were sacrificed at the 8th week after medication. The 24-h urinary protein excretion (24 h-UP) and blood biochemical indices were determined. Renal tissues were collected for pathological changes under light and electron microscopes. Expressions of fibronection (FN),collagen Ⅳ (COL-Ⅳ), and osteopont (OPN) in renal tissues were detected by immunohistochemistry. mRNA levels of transforming growth factor-beta 1 (TGF-β1), tissue inhibitor of metalloproteinase-1 (TIMP-1), and plasminogen activator inhibitor-1 (PAl-1) were measured by fluorescent Real-time PCR. Results When compared with the model group,24 h-UP, blood urea nitrogen (BUN), and serum creatinine (SCr), and blood lipids levels were significantly lowered in the X group. The mesengial matrix percentage was less in the X group than in the M group. Renal FN, COL-Ⅳ, and OPN expressions more significantly decreased in the X group than in the M group. Similarly mRNA expressions of TGF-β1 TIMP-1, PAl-1 in renal tissues obviously decreased. Conclusion Xinganbao Capsule could exert its renal protective action possibly through reducing the urinary protein excretion, correcting lipid metabolic disturbance, inhibiting excessive accumulation of extracellular matrix, decreasing the expression of fibrosis factors, and improving the pathological damage of kidneys in the AIN rat model.关键词
阿霉素肾病/肾纤维化/心肝宝胶囊/贝那普利Key words
adriamycin induced nephropathy/ renal fibrosis/ Xinganbao Capsule/ Benazepril引用本文复制引用
刘茂静,王娜,亓敏,梁素忍,甄军晖,冯进波,胡昭..心肝宝胶囊对阿霉素肾病大鼠的肾脏保护作用[J].中国中西医结合杂志,2011,31(5):690-695,6.基金项目
国家"十一五"科技支撑计划项目(No.2007BAI04B10) （No.2007BAI04B10）
