中国输血杂志2011，Vol.24Issue(4)：323-326,4.
2种国产血小板滤器滤除白细胞对体外血小板功能的影响
The effect of two types of home-make leukocyte filters on the in vitro function of platelet concentrates
摘要
Abstract
Objective To evaluate the changes in platelet function after filtering leukocytes with two types of leukocytes filter for PCs.Methods PCs was prepared from 400 ml fresh whole blood by platelet - rich plasma (PRP) method.Six bags of ABO -matched PCs were pooled,and then filtered with two types of domestic filters (namely group A and B),10 samples respectively.The counted numhers of platelet and leukocyte, pH, CD62p expression (％), platelet aggregation and hypotonic shock response (HSR) were detected before and after filtering.Results After filtering leukocytes,the platelet recovery, the residual leukocytes and the pH, group A and B respectively, were (87.01 ± 3.47) ％ vs (87.88 ± 4.77 ) ％,(0.95 ±0.90) × 106 vs(0.45 ±0.58) × 106 and (7.13 ±0.13) vs ( 6.80 ±0.26).The CD62p expression (％) ,platelet aggregation and hypotonic shock response (HSR) of group A before and after filtering were (8.06 ±4.11)％ vs (8.21±4.50)％, (70.55 ±27.21 )％ vs (71.63 ±32.24)％ and (68.14 ± 10.13)％ vs (69.18 ±9.38)％ ,while those of group B were(10.34±3.26)％ vs (10.47 ±2.42)％,(56.30 ±18.43)％ vs (59.49±19.15)％ and(75.73 ±5.50) vs (73.74 ± 6.52) ％, respectively.There were no significant difference ( P ＞ 0.05 ) between these items before and after filtering.Conclusion Filtration with two types of domestic leukocyte filters didn't activate platelet and had no influence on platelet aggregation and HSR.Besides ,both the platelet recovery and residual leukocytes met the requirements of Chinese standards.These two types of domestic leukocyte filters could be applicable to the leukocyte removing for PCs.关键词
浓缩血小板/白细胞滤器/CD62p/血小板聚集/血小板低渗休克Key words
Platelet concentrates/ Leukocyte filter/ CD62p/ Platelet aggregation/ Hypotonic shock response分类
医药卫生引用本文复制引用
王红,刘嘉馨,雷宇,曹晔,钟锐,谢月娜,何语良,刘曦,袁理..2种国产血小板滤器滤除白细胞对体外血小板功能的影响[J].中国输血杂志,2011,24(4):323-326,4.基金项目
四川省科技支撑计划2008SZ0101 （）
