新疆农业科学2011，Vol.48Issue(4)：599-605,7.
新疆春小麦品种资源1BL/1RS易位和Bx7OE基因的分子检测
Detection of 1BL/1RS Translocation and Bx7OE Gene by Using STS Marker in Xinjiang Spring wheat Germplasm Resources
摘要
Abstract
[ Objective]The aim of this study was to provide useful materials and method of marker - assisted selection ( MAS) for improvement of wheat gluten quality in Xinjiang. [ Method ] TotaLly, 162 Xinjiang spring wheat germplasm resources were used to investigate the distribution of wheat - rye 1BL/1RS translocation and Bx7OE gene by using four STS markers, meanwhile to verify the feasibility of four markers. [Result] The results showed that among the 162 spring wheat germplasm, 24 (14. 8 ％ ) , 3( 1 . 9 ％ ) were 1BL/1RS translocation lines and cultivar wit Bx7OE gene, respectively. The frequencies of 1BL/1RS translocation and Bx7OE gene were significantly different in landraces, introduced cultivars and bred cultivars in Xinjiang, and their frequencies were as follows:7.5 ％ and ％ , 24.4％ and 6 . 7 ％ , 14. 1 ％ and ％ , respectively. [ Conclusion] The frequencies of 1BL/1RS translocation and Bx7OE gene in Xinjiang spring wheat cultivars were lower than that of other province, which was directly related to parent cultivars. The four STS markers had characteristics of good stability, high efficiency and reliability for amplifying wheat genomic DNA, thus they can be used as a molecular tool for marker - assisted selection( MAS)in wheat breeding programs targeting for wheat gluten strength .gene; STS marker;Xinjiang关键词
春小麦/1BL/1RS易位/Bx7OE基因/STS标记/新疆Key words
Xinjiang spring wheat/ 1BL/1RS/ Bx7OE分类
农业科技引用本文复制引用
王亮,穆培源,徐红军,崔凤娟,李士磊,冶婷,李卫华..新疆春小麦品种资源1BL/1RS易位和Bx7OE基因的分子检测[J].新疆农业科学,2011,48(4):599-605,7.基金项目
石河子大学研究生创新基金项目(YJCX2010-Y04) （YJCX2010-Y04）
新疆农垦科学院青年科学基金项目(YQJ2009-09) （YQJ2009-09）
新疆生产建设兵团科技支疆计划项目(2008ZJ10) （2008ZJ10）
新疆生产建设兵团科技攻关计划(2009GG05) （2009GG05）
