安徽医科大学学报2011，Vol.46Issue(9)：950-952,981,4.
HCMV pp65抗原血症和UL83基因检测对肾移植术后HCMV感染的诊断价值
Comparison of pp65 antigenemia assay and polymerase chain reaction for UL83 DNA for detection of HCMV in renal transplant patients
摘要
Abstract
Objective To compare the results obtained using HCMV pp65 antigenemia assay and polymerase chain reaction for detecting HCMV UL83 DNA on the peripheral blood leukocytes which collected from recipients after kidney transplantation. Methods HCMV pp65 antigenemia assay and polymerase chain reaction were respectively used for detecting HCMV UL83 DNA on the peripheral blood leukocytes which collected from recipients of whom regularly follow-up after renal transplantation. Results There were 13 samples positive for pp65 antigenemia assay in the 77 patients, and the positive rate was 16. 9％ . There were 15 samples positive for polymerase chain reaction of detecting HCMV UL83 DNA . and the positive rate was 19. 5％ . Among 77 patients, there were 16 patients diagnosed cytomegalovirus infection in clinical. seven of whom were symptomatic. The concordance between PCR and pp65 antigenemia assay was 94％ . The positive concordance was 75％ . and the negative concordance was 94％.Conclusion It is more accurate and objective to use pp65 antigenemia assay and polymerase chain reaction together to monitor active cytomegalovirus infection and the curative effect.关键词
巨细胞病毒/巨细胞病毒感染/诊断/肾移植/聚合酶链反应Key words
cytomegalovirus/cytomegalovirus infections/diagnosis/kidney transplantation/polymerase chain reaction分类
医药卫生引用本文复制引用
谢栋栋,林政,施浩强,吴琼,王明丽,陈磊,唐亮,廖贵益,于德新..HCMV pp65抗原血症和UL83基因检测对肾移植术后HCMV感染的诊断价值[J].安徽医科大学学报,2011,46(9):950-952,981,4.基金项目
安徽医科大学科研基金项目(编号:2008kj17) （编号:2008kj17）
