中国动脉硬化杂志2011，Vol.19Issue(2)：100-104,5.
Toll样受体9对巨噬细胞脂周素2表达的影响
The Effect of ODN1826, a TLR9 Ligand, on the Expression of Perilipin2 in Macrophages
范斌 1谷剑秋 2张锦 2生山祥一郎3
作者信息
- 1. 中国医科大学附属盛京医院神经内科,辽宁省沈阳市,110004
- 2. 中国医科大学附属第一医院内分泌科,辽宁省沈阳市,110004
- 3. 日本九州大学生体防御医学研究所免疫病态学分野,874-0838,日本
- 折叠
摘要
Abstract
Aim To investigate the effect of ODN1826, a TLR9 ligand, on perilipin 2 expression in macrophages.Methods Real-time PCR and Western blotting were performed to detect perilipin 2 expression.Transient transfection and luciferase assay were employed to measure the perilipin 2 promoter activity.Results ODN1826 significantly induced perilipin 2 expression in a dose and time dependent manner in macrophages.ODN1826 markedly enhanced perilipin 2 promoter activity, and this process requires Ets/AP-1 activity.Conclusions TLR9 signal pathway stimulated perilipin 2 expression in macrophages, in addition, ODNl826-induced perilipin 2 promoter activity requires Ets/AP-1 activity.关键词
Toll样受体/泡沫细胞/动脉粥样硬化/脂周素2Key words
Toll-like Receptor/ Foam Cell/ Atherosclerosis/ Perilipin 2分类
医药卫生引用本文复制引用
范斌,谷剑秋,张锦,生山祥一郎..Toll样受体9对巨噬细胞脂周素2表达的影响[J].中国动脉硬化杂志,2011,19(2):100-104,5.
