中国中西医结合杂志2011，Vol.31Issue(6)：807-810,4.
黄芪甲苷对内皮祖细胞氧化损伤的影响
Effect of Astragaloside against the Oxidative Damage on Endothelial Progenitor Cells
摘要
Abstract
Objective To observe the effect of astragaloside on oxidative low-density lipoprotein (Ox-LDL)mediated oxidative damage of endothelial progenitor cells (EPCs). Methods Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density gradient centrifugation, and EPCs were identified by flow cytometry. Adherent cells were collected after seven-day incubation and randomly divided into the normal control group, the Ox-LDL group (as the model group, at the dose of 100 μg/mL), the low, middle, and high astragaloside groups (with 100 μg/mL Ox-LDL plus 2, 10, and 50 μg/mL astragaloside). Twenty-four h later, the proliferation and adhesion capabilities of EPCs were observed using MTT colorimetry and the adhesion capability detection. Levels of superoxide dismutase (SOD) and malonaldehyde (MDA) in the cell supemate of each group were determined. Results After Ox-LDL damage, the proliferative and adhesive capacities of EPCs were significantly injured (53 ±8 rs42 ±6, 0. 49 ±0. 12 vsO. 37 ±0. 02, both P＜0. 05). The SOD content obviously decreased (21.95 ±1.43 vs 14. 76 ±3. 99, P＜0. 01 ), the MDA content obviously increased (3. 72 ±0. 30 vs 5. 57 ±0. 64, P＜0. 01 ). After intervened by astragaloside for 24 h, the proliferative and adhesive capacities of EPCs were significantly improved. The SOD contents of astragaloside intervention groups were obviously improved and the MDA content obviously lowered. Conclusions Astragaloside showed significant protection on Ox-LDL damaged EPCs. Its mechanism might be correlated with antioxidative damage.关键词
黄芪甲苷/内皮祖细胞/氧化低密度脂蛋白/超氧化物歧化酶/丙二醛Key words
astragaloside/ endothelial progenitor cell/ oxidative low-density lipoprotein/ superoxide dismutase/ malonaldehyde引用本文复制引用
季亢挺,唐疾飞,柴俊德,陈邢玉,林加锋,杨鹏麟..黄芪甲苷对内皮祖细胞氧化损伤的影响[J].中国中西医结合杂志,2011,31(6):807-810,4.基金项目
浙江省教育厅课题(No.Y201017426) （No.Y201017426）
浙江省卫生厅基金项目(No.2007A142) （No.2007A142）
温州市科技局项目(No.H20080027) （No.H20080027）
