针刺研究2011，Vol.36Issue(2)：90-94,5.
"嗅三针"对阿尔茨海默病大鼠海马毒覃碱型受体的影响
Effect of"Xiusanzhen" on Hippocampal Muscarinic Cholinergic Receptor Activity in Alzheimer Disease Rats
摘要
Abstract
Objective To explore the effect of "Xiusanzhen" [electroacupuncture (EA) of bilateral "Yingxiang" (LI20) and "Yintang" (EX-HN 3)] on activities of hipppocampal muscarinic cholinergic receptor (mAChR) and the involved neural path in Alzheimer Disease(AD)rats. Methods Forty Sprague Dawley rats were randomly divided into normal control, model,olfactory nerve severing (ONS)-EA of "Xiusanzhen" (ONS-EA) and EA of "Xiusanzhen" (EA) groups. AD model was established by intrahippocampal injection (AP:3.5 mm, L:2 mm, H: -2.8 mm) of Aβ1-40 starch-like peptide ( 10 μg/2 μL) under the aid of a microsyringe installed in a brain stereotaxis instrument. For rats of the ONS-EA group, the olfactory nerve was severed by using a surgical knife after drilling a hole on the skull (5 mm anterior to the bregma, L, R: 2 mm). The mAChR density, and its maximum binding capacity (Bmax) and dissociation constant (Kd) of the hippocampus tissue were measured by using radio-ligand binding analysis and Lowry's microamount protein assay. Results In comparison with the normal control group, the hippocampal mAChR density and its Bmax in the model group were decreased remarkably(P＜0.05), while the Kd of M-receptor in the model group was increased remarkably (P＜0.05). In comparison with the model group, hippocampal mAChR density and its Bmax in the EA group were up-regulated obviously(P＜0.05), while the Kd of mAChR in the EA group was down-regulated significantly (P＜0.05). No significant differences were found between the model and ONS-EA groups in mAChR density and its Bmax and Kd (P＞0. 05). Conclusion "Xiusanzhen"-EA can effectively up-regulate hippocampal mAChR density and Bmax and down-regulate Kd of M-receptor of hippocampus in AD rats, which is dependent on the intact olfactory nerve pathway.关键词
阿尔茨海默病/嗅三针/电针/胆碱能M受体/海马Key words
AIzheimer Disease/ Xiusanzhen/ Electroacupuncture/ Muscarinic cholinergic receptor/ Hippocampus分类
医药卫生引用本文复制引用
杨晓航,刘智斌,牛文民,牛晓梅,王渊.."嗅三针"对阿尔茨海默病大鼠海马毒覃碱型受体的影响[J].针刺研究,2011,36(2):90-94,5.基金项目
国家自然科学基金资助(No.30973792) （No.30973792）
