安徽农业科学2011，Vol.39Issue(14)：8617-8618,8652,3.
鸡新城疫-传染性支气管炎-H9亚型禽流感三联灭活疫苗的制备及检验
Preparation and Examination of Inactivated Emulsion Vaccine against Newcastle Disease,Infectious Bronchitis and H9N2 Subtype Avian Influenza
张建伟 1李林 1章振华 1景小冬 1郑小兰 1姜北宇1
作者信息
- 1. 北京市农林科学院畜牧兽医研究所,北京,100097
- 折叠
摘要
Abstract
[Objective] To prepare inactivated emulsion vaccine against Newcastle disease,infectious bronchitis and H9N2 subtype avian influenza. [Method] Antigen fluid of Newcastle disease virus (NDV) LaSota strain,infectious bronchitis virus (IBV) M41 strain and H9N2 subtype avian influenza virus (AIV) WD strain was prepared by propagation in chicken embryos,respectively. The antigen fluid was concentrated with FILTRON Cassette ultrafiltration system and activated by formalin. The antigen fluid of NDV,IBV and AIV was mixed at a volume ratio of 1∶ 1∶1. Then the mixture was emulsified by Span-80 and Tween -80 and added white oil No. 10 as adjuvant. The sterility and physical characteristics of the prepared NDV-IBV-AIV combined vaccine were detected. [ Result ] The three batches of NDV-IBV-AIV combined vaccine were germfree,milky white,with Water-in-Oil pattern and with viscosity of 6.3 -6.8. The water and oil were not separated after standing at 37 ℃ for 21 d or centrifugation. [ Conclusion] The three batches of NDV-IBV-AIV combined vaccine were germ-free and reached the standard for physical characteristics of vaccines.关键词
鸡新城疫/传染性支气管炎/禽流感/灭活疫苗Key words
Newcastle disease/ Infectious bronchitis disease/ Avian ínfluenza disease/ Inactivated vaccine分类
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张建伟,李林,章振华,景小冬,郑小兰,姜北宇..鸡新城疫-传染性支气管炎-H9亚型禽流感三联灭活疫苗的制备及检验[J].安徽农业科学,2011,39(14):8617-8618,8652,3.
