中国兽医科学2011，Vol.41Issue(4)：403-408,6.
大黄素对SLT-Ⅱe诱导大鼠肠黏膜微血管内皮细胞分泌细胞因子的影响
Effects of emodin on the secretion of NO,ET-1,PG12,TXA2,P-selectin and sICAM-1 in rat intestinal microvascular endothelial cells induced by SLT-Ⅱe
摘要
Abstract
The concentrations of NO,ET-1,PGI2,TXA2, P-selectin and sICAM-1 which were induced by SLT-Ⅱ e in intestinal microvascular endothelial cells of rats were determined to study the functional mechanism of emodin on edema disease of swine.The cultured RIMECs in vitro were divided into control group,SLT-Ⅱ e group,test 1 group,test 2 group and test 3 group.The concentrations of NO,ET-1 ,PGI2,TXA2,P-selectin and sICAM-1 in cell supernatant which were cultivated for 3 h,6 h,9 h and 12 h were detected by ELISA,respectively.In result, 10 μg/mL emodin had reduced the secretion of NO, ET-1 and Pselectin remarkably.The secretion of PGI2,TXA2 and sICAM-1 were not reduced by 1 μg/mL, 5 μg/mL and 10 μg/mL emodin.In conclusion,emodin could reduce the overfull secretion of NO, ET-1 and P-selectin in IMECs of rat, increase NO/ET-1 ratio, relive intestinal microcirculation obstruction, and alleviate the inflammation response.关键词
SLT-Ⅱe/肠黏膜微血管内皮细胞/NO/ET-1/PG12/TXA2/P-选凝素/sICAM-1/大黄素Key words
SLT- Ⅱ e/ RIMEC/ NO / ET-1/ PGI2/ TXA2/ P-selectin/ sICAM-1/ emodin分类
农业科技引用本文复制引用
周宏超,刘钟杰,穆祥..大黄素对SLT-Ⅱe诱导大鼠肠黏膜微血管内皮细胞分泌细胞因子的影响[J].中国兽医科学,2011,41(4):403-408,6.基金项目
国家自然科学基金项目(30671543) （30671543）
北京市自然科学基金项目(6021001) （6021001）
北京市属市管高等学校人才强教计划项目(BAHED) （BAHED）
