中国兽医科学2011，Vol.41Issue(4)：418-421,4.
乳乃康有效成分阿莫西林在牛奶中残留量的动态变化
Dynamic changes of the residual quantity of amoxicillin of Runaikang drug in the cow milk
摘要
Abstract
Eight quarters in four healthy cows, which were LMT-negative, were selected to examine dynamic changes of the residual amount of amoxicillin in the cow after Runaikang was administered. Using reversed-phase high performance liquid chromatography(RP-HPLC), the changes of amount of amoxicillin,the main ingredients in the Runaikang,were examined for 7 days in succession after the drug was given and the time of residual in the bodies was evaluated. Samples were extracted by phosphate buffer, precipitated proteins with trichloroacetic acid(TCA) solution, cleaned up with and enriched by solid phase extraction (SPE) ,and derivatised with 1,2,4-triazole mercury( Ⅱ ) reagent. The limit of detection was below 0.03 mg/L. In result,the average amount of residual was 12.49 μg/L at hour 24,5.92 μg/L at hour 48,2.51 μg/L at hour 72 and 0.59 μg/L at hour 96 post-Runaikang-withdrawl. The elimination curve in the experiment indicated that the discard milk period should be 3 days.关键词
乳乃康/乳牛/高效液相色谱/阿莫西林/残留Key words
Runaikang / cow / high performance liquid chromatography/ amoxicillin / residue分类
农业科技引用本文复制引用
郁杰,王一萍,葛竹兴,洪伟鸣,邢晓玲,王妲妲,王付景..乳乃康有效成分阿莫西林在牛奶中残留量的动态变化[J].中国兽医科学,2011,41(4):418-421,4.基金项目
江苏省泰州市2008年农业科技计划项目(TS0802) （TS0802）
