中国兽医科学2011，Vol.41Issue(4)：342-346,5.
表达乙型脑炎病毒PrM-E基因慢病毒载体的构建
Construction of lentiviral vector expressing PrM-E gene of Japanese encephalitis virus
摘要
Abstract
PrM-E gene was amplified from Japanese encephalitis virus(JEV) by RT-PCR and cloned into the transfer plasmid pHR-IRES-EGFP. The recombinant plasmid pHR-PrM-E was then co-transfected into 293FT cells with packaging vector pCMVΔ8.2 and envelope vector pVSV/G. At 48 hour post-transfection,the supernatant was harvested and transduced 293FT cells. Subsequently, the expression of target protein was examined by indirect immunofluorescence assay and Western-blot. The results demonstrated that the lentiviral particles were successfully packaged by co-transfection of these three plasmids and could transduce 293FT cells efficiently. Also PrM-E gene was expressed correctly in the transduced cells,which provided the experimental basis for the development of novel JEV vaccines in future.关键词
乙型脑炎病毒/慢病毒载体/PrM-E基因Key words
Japanese encephalitis virus/ lentiviral vector/ PrM-E gene分类
农业科技引用本文复制引用
陈龙,杨晓红,徐敏,朱碧波,陈焕春,曹胜波..表达乙型脑炎病毒PrM-E基因慢病毒载体的构建[J].中国兽医科学,2011,41(4):342-346,5.基金项目
农业公益性行业科研专项(200803015) （200803015）
