中国兽医科学2011，Vol.41Issue(4)：347-350,4.
副猪嗜血杆菌ERIC-PCR分型方法的建立及其应用
Establishment and application of ERIC-PCR method for genotyping of in Haemophilus parasuis
摘要
Abstract
A pair of primers were designed according to the ERIC sequence. The reaction system including the concentrations of templates, primers, DNA polymerase, dNTPs and the annealing temperature were explored and optimized for development of an ERIC-PCR method. Fingerprints of clinical isolates were analyzed by the established ERIC-PCR method for genotyping of Haemophilus parasuis. In result,the optimal concentration of primers was from 0.166 μmol/L to 0. 333 μmol/L, the concentration of DNA polymerase was 1.0 U/μL. The concentration of template DNA was 8 ng/μL, and dNTPs was 200μmol/L in content. Using the method,ERIC-PCR fingerprinting of 13 isolates compared with the 15 standard serumbased fingerprint could be distinguished six kinds of serotypes. These results were consistent with that of serological agar diffusion testing method. The results showed that the established ERIC-PCR technique for genotyping of H. parasuis had good reproducibility and high comparability.关键词
副猪嗜血杆菌/肠杆菌科基因间重复序列为引物的聚合酶链反应/分型Key words
Haemophilus parasuis/ ERIC-PCR/ genotyping分类
农业科技引用本文复制引用
郑念广,谭实勇,陈瑞爱,何玲,夏芳,罗满林..副猪嗜血杆菌ERIC-PCR分型方法的建立及其应用[J].中国兽医科学,2011,41(4):347-350,4.基金项目
广东温氏食品集团有限公司2008年度第八批科研项目(C080921) （C080921）
