中国兽医科学2011，Vol.41Issue(4)：387-391,5.
猪细小病毒抗体IPMA检测方法的建立及应用
Establishment and application of immunoperoxidase monolayer assay for detection of the antibody against porcine parvovirus
摘要
Abstract
An immunoperoxidase monolayer assay (IPMA) was developed to detect antibody against porcine parvovirus(PPV). The reaction conditions, specificity, sensitivity, and reproducibility of IPMA were optimized,evaluated,and compared with that of HI and ELISA. In the optimized IPMA, the virus was diluted at 1: 100 after 48 hours,HRP-SPA was diluted at 1: 1 000,and swine serum samples were diluted at 1: 100. There was no cross-reaction with reference sera against other porcine viruses. The sensitivity test showed that the positive sera could be detected at 1 : 1 600. Reproducibility tests revealed that the established IPMA had good reproducibility. The IPMA method had 96.0％ and 98.0％ correlation with HI and ELISA method respectively. About 100％ positive rate could be achieved by the assay for PPV-immunized porcine sera on the 6th week post-inoculation. A total of 400 serum samples were examined using this method. The positive rate was 86.5 ％. This method offered a convenient tool for the epidemiological investigation of PPV infections and quick evaluation of antibodies against the virus.关键词
猪细小病毒/免疫过氧化物酶单层细胞试验/抗体检测Key words
porcine parvovirus(PPV) /immunoperoxidase monolayer assay(IPMA)/ antibody detection分类
农业科技引用本文复制引用
刘杉杉,赵亚荣,张超范,吕超超,胡泉博,崔尚金..猪细小病毒抗体IPMA检测方法的建立及应用[J].中国兽医科学,2011,41(4):387-391,5.基金项目
国家自然科学基金项目(31001069) （31001069）
大北农企业横向课题(2010JS) （2010JS）
