临床与实验病理学杂志2011，Vol.27Issue(7)：746-750,5.
皮肤瘢痕及瘢痕癌组织中cyclin A、p21WAF1的表达及意义
Expressions and significance of cyclin-A and p21WAF1 in skin scar and skin scar carcinoma
摘要
Abstract
Purpose To study the expressions and significance of cyclin A and p2IWAF1 in skin scar and skin scar carcinoma. Methods The cases of pathological skin scar and skin scar carcinoma were studied to compare with normal skin tissues. The expression of cyclin A and p21WAF1 proteins were detected by the SP immunohistochemical method. The expression of cyclin A mRNA,p21WAF1 mRNA were detected by in situ hybridization. The expression ( optical density and positive area ) of cyclin A, p21WAF1 proteins and cyclin A mRNA、p21 WAF1 mRNA were detected respectively by computerized image-analysis in normal skin epidermis, pathological skin scar epithelium and scar carcinoma tissues. All data was input into the computer and statistically analyzed with SPSS ( 16. 0 ) software. Results ( 1 ) cyclin A and its mRNA showed strong positive expression in scar carcinoma tissues,negative or weakly positive expression in normal skin epidermis and pathological skin scar epithelium. Compared with normal skin and skin scar, the expression ( optical density and positive area ) of cyclin A and its mRNA in scar carcmoma were statistically significant ( P ＜ 0. 01 ). No statistic differences of their expression were measured between normal skin epidermis and pathological skin scar epithelium. ( 2 )p21WAF1 protein and its mRNA showed strong positive expression in scar carcinoma tissues, negative or weakly positive expression in normal skin epidermis and pathological skin scar epithelium. Compared with normal skin and skin scar, the expression ( optical density and positive area ) of cyclin A and its mRNA in scar carcinoma were statistically significant ( P ＜ 0. 01 ). No statistic differences of their expression were measured between normal skin epidermis and pathological skin scar epithelium. ( 3 )Correlated analysis showed that there was positive correlation between the expression levels of cyclin A and cyclin A mRNA,p21WAF1 and p21WAF1 mRNA,cyclin A and p21WAF1 proteins, cyclin A mRNA and p21WAF1 mRNA in skin scar carcinoma tissues. Conclusions ( 1 )The high expression of cyclin A and cyclin A mRNA may be related to the occurrence of skin scar carcinoma. ( 2 )There was the abnormal expression of cyclin A and p21WAF1 not only on protein levels but also on gene levels in skin scar carcinoma tissues.( 3 )The high expression of p21WAF1 protein and p21WAF1 mRNA may be related to the feedhack mechanism of cell cycle regulation in scar carcinoma tissues.关键词
皮肤瘢痕/瘢痕肿瘤/肿瘤变/cyclin A/p21WAF1Key words
skin scar/ scar neoplasms/ cancerization/ cyclin A/ p21WAF1分类
医药卫生引用本文复制引用
周开梅,郭瑞珍,陈世玖..皮肤瘢痕及瘢痕癌组织中cyclin A、p21WAF1的表达及意义[J].临床与实验病理学杂志,2011,27(7):746-750,5.基金项目
贵州省攻关项目(2010-3080) （2010-3080）
