安徽农业科学2011，Vol.39Issue(8)：4540-4542,3.
剑麻皂素的分离纯化工艺研究
Study on Technology of Isolation and Purification of Tigogenin
摘要
Abstract
[ Objective ] The research aimed to study the technology of extraction,isolation and purification of tigogenin from Agave sisalana Perr.ex Engelm. [ Method ] The crude extracts of tigogenin were extracted by using conventional heating reflux extraction method with sulphuric acid,isolated and purified by macroporous resin,and the optimum technology was determined. [ Results] It was better to isolate tigogenin by using D101 resin. The recovery rate and purity were respectively 79.1％ and 71.6％ under the optimum conditions,which were isolated and purified at the speed of 0.6 ml/min with 100 ml of 70％ alcohol as eluent. [ Conclusion] The experimental method was feasible for industrial production of tigogenin due to reasonable technique method and lower raw materials,关键词
剑麻皂素/大孔树脂/提取/分离/高效液相色谱法Key words
Tigogenin,Macroporous resin,Extraction,Isolation and purification,HPLC分类
农业科技引用本文复制引用
周寅,张梦宇,白杨,贾桂云..剑麻皂素的分离纯化工艺研究[J].安徽农业科学,2011,39(8):4540-4542,3.基金项目
海南师范大学国家大学生创新实验计划资助项目(00203012702). （00203012702）
