昆明医学院学报2011，Vol.32Issue(4)：16-19,4.
TRB3在同型半胱氨酸抑制内皮细胞增殖中的作用研究
TRB3 Mediates Homocysteine-induced Inhibition of Endothelial Cells Proliferation
摘要
Abstract
Objective To investigate the effect of TRB3 on homocysteine-induced inhibition of endothelial cells proliferation. Methods DNA synthesis in cells was determined by using [3H]-thymidine incorporation.The expression of tested genes was detected by real-time PCR. The phosphorylation of proteins was measured by Westren blotting. Results (1) Homocysteine inhibited the proliferation of human umbilical vein endothelial cells (HUVECs). (2) Homocysteine induced endoplasmic reticulum stress, and up-regulated the expression of CHOP and ATF4. (3) Homocysteine up-regulated the expression of TRB3, resulting in dephosphorylation of Akt. Conclusion TRB3 is a critical molecule in homocysteine-mediated inhibition of proliferation in HUVECs.关键词
同型半胱氨酸/内皮细胞/细胞增殖/TRB3/AktKey words
Homocysteine/ Endothelial cell/ Cell proliferation/ TRB3/ Akt分类
医药卫生引用本文复制引用
单妍,田兴亚,张伟,梁璇,刘佳,邰文琳..TRB3在同型半胱氨酸抑制内皮细胞增殖中的作用研究[J].昆明医学院学报,2011,32(4):16-19,4.基金项目
云南省教育厅及昆明医学院海源学院基金资助项目(JYT0901H) （JYT0901H）
