昆明医学院学报2011，Vol.32Issue(4)：26-29,4.
不同冷冻载体对小鼠卵巢组织玻璃化冻存的影响
The Effect of Different Carriers on Mice Ovarian Tissue Vitrification
摘要
Abstract
Objective To observe the effect of different carriers on mouse ovarian tissue vitrification.Methods Ovaries tissue slices from mouse about 9 ～ 12 weeks were vitrified by close straw, open straw or cryoloop.After thawed, autotransplantations were performed and two pieces of ovarian tissue slices were respectively inserted under the kidney capsule of two sides in each mouse.The survival rate of grafts, histological structure, follicular density and serum E2 level in each group were detected after autotransplantation for 21 days.The effect of different freezing carriers on viability of frozen-thawed ovarian tissue was compared.Results The survival rate of grafts after 21 days autotransplantation in close straw group, open straw group or cryoloop group was respectively 78.5％, 78.5％ and 84.6％; the number of follicles in per high power field was 4.0 ± 0.8, 4.5 ±0.7 and 4.6 ± 0.9; the serum E2 level of mouse was 10.4 ± 0.8 pg/mL, 11.0 ± 1.6 pg/mL and 12.5 ± 1.1 pg/mL.Among them, there was significant difference between cryoloop group and close straw group in the survival rate of graft and the serum E2 level (P ＜ 0.05 ).Conclusions The survival rate of graft and estradiol secretion can be improved by using cryoloop as vitrification carrier.关键词
小鼠/卵巢组织/玻璃化冻存/自体移植Key words
Mouse/ Ovary/ Vitrification/ Autotransplantation分类
医药卫生引用本文复制引用
李跃萍,黄元华,闫庆峰..不同冷冻载体对小鼠卵巢组织玻璃化冻存的影响[J].昆明医学院学报,2011,32(4):26-29,4.基金项目
海南省自然科学基金资助项目(30866) （30866）
