南方农业学报2011，Vol.42Issue(5)：509-511,3.
广西北部蔗区甘蔗黄叶病调查
Investigations on sugarcane yellow leaf disease in northern sugarcane growing areas of Guangxi
摘要
Abstract
[Objective]The present study was conducted to find out the occurrence of sugarcane yellow leaf virus (SCYLV) disease in the north sugarcane growing areas of Guangxi, and to provide scientific basis for healthy seedcane production of sugarcane. [Method ]Sugarcane leaf samples with different stages of symptoms were collected from Liuzhou suburbs (8 samples), LiucheNg (7 samples), Luzhai (4 samples) and Yizhou (6 samples) areas of Guangxi. The virus was detected using one-step RT-PCR method with SCYLV specific primers (P1 :5＇-AATCAGTGCACACATCCGAG-3'/ P2: 5 ＇-GGAGGGTCGCCTACCTATTI-3＇). The amplified cDNA products were sequenced. [ Result]Six out of 8 varieties (occupying about 75％ ) were found infected with SCYLV in Liuzhou suburbs and the rate of SCYLV infection in other three towns, viz., LiucheNg, Luzhai and Yizhou were 100.0, 50.0 and 66.7％, respectively. In addition, the varieties,GT21, LiucheNg 03-182 and ROC16 were found infected by SCYLV in the tested areas. The major cultivated sugarcane variety ROC22 also gave positive results of SCYLV test in Liuzhou and Yizhou areas. [Conclusion]Sugarcane yellow leaf disease was found to occur in northern areas of Guangxi, efforts must be done to develop proper measures to control the spread of this disease.关键词
甘蔗/黄叶病/调查/广西北部蔗区Key words
sugarcane/ sugarcane yellow leaf virus (SCYLV)/ investigation/ northern sugarcane of Guangxi分类
农业科技引用本文复制引用
颜梅新,黄伟华,黄诚华..广西北部蔗区甘蔗黄叶病调查[J].南方农业学报,2011,42(5):509-511,3.基金项目
现代农业产业技术体系建设专项资金项目(nycytx-024-01-07) （nycytx-024-01-07）
广西自然科学基金项目(2010GXNSFB013026) （2010GXNSFB013026）
广西农业科学院基本科研业务专项项目(201004) （201004）
广西甘蔗研究所基本科研业务专项项目(G2010010) （G2010010）
