安徽医科大学学报2011，Vol.46Issue(7)：627-630,4.
青春期氰戊菊酯暴露对小鼠大脑皮层性激素的影响
Effects of fenvalerate exposure during puberty on gonadal hormone in cerebral cortex of mice
摘要
Abstract
Objective To explore the effects of fenvalerate exposure during puberty on gonadal hormone in mice and its molecular mechanisms. Methods Sixty male and female mice in postnatal day ( PND ) 28 were randomly divided into three groups : the control group and fenvalerate ( 7. 5 mg/kg and 30 mg/kg ) group. Pubertal male and female mice in treatment groups were treated with fenvalerate ( 7. 5,30 mg/kg ) by gavage daily from PND28 to PND56,while the controls received corn oil. After two weeks, chose two mice from every group, then these mice were sacrificed. Cerebral cortex was excised. The remained mice were killed after four weeks , whose cerebral cortex was also excised. Protein expression of StAR,testosterone synthetic enzymes, androgen receptor ( AR ), two estrogen receptors( ERa and ERβ )in cerebral cortex were analyzed by Western blot. The content of testosterone ( T ) and estradiol ( E2 ) in cerebral cortex were measured by radioimmunoassay ( RIA ). Results Pubertal fenvalerate ( 30 mg/kg ) exposure markedly decreased T and E2 in cerebral cortex of male mice, whereas the level was significantly elevated in females. In agreement with above results, pubertal fenvalerate exposure obviously downregulated protein expression of 17β-HSD in cerebral cortex of males , and upregulated 17β-HSD expression in cerebral cortex of females. Additionally, pubertal fenvalerate exposure for two weeks and four weeks significantly enhanced the protein expression of AR in cerebral cortex of females, whereas in males, the protein expression of AR and ERβ in cerebral cortex was markedly upregulated in pubertal fenvalerate exposure for four weeks. Conclusion Pubertal fenvalerate exposure disrupts T and E2 synthesis and AR expression in cerebral cortex of mice. These alterations of steroids and the receptors in cerebral cortex might be detrimental to neurobehavioral development.关键词
青春期/大脑皮质/睾酮/雌二醇Key words
puverty/cerebral cortex/testoerone/estradiol分类
医药卫生引用本文复制引用
刘萍,孟秀红,王华,姬艳丽,陈远华,张程,徐德祥..青春期氰戊菊酯暴露对小鼠大脑皮层性激素的影响[J].安徽医科大学学报,2011,46(7):627-630,4.基金项目
国家自然科学基金(编号:30371667、30572223、3067 1786) （编号:30371667、30572223、3067 1786）
教育部重点项目(编号:208060) （编号:208060）
