重庆医学2011，Vol.40Issue(21)：2084-2086,3.DOI:10.3969/j.issn.1671-8348.2011.21.002
Alphastatin多肽对胶质瘤血管生成拟态的抑制作用及其相关机制
The effection and the mechanism of alphastatin supressing the vasculogenic mimicry formation of glioma
摘要
Abstract
Objective To investigate the effection and the mechanism of alphastatin supressing the vasculogenic mimicry formation of glioma.Methods MTT,transwell and three-dimentional culture were used to detect the proliferation, migration and tubule formation of SHG44.The expression of VEGF, EphA2 and MMP2 was detected by western blot.Results The number of cell migration in control group was 178.71± 18.81 , significantly higher than that in experimental group(142.57±12.12 , 92.71± 17.68 ,30.00±7.±72) (P＜0.01).The number of vasculogenic mimicry was 56.80±12.21 , also significantly higher than that in experimental group(47.71±10.58 ,18.86±8.40 , 8.43±5.62) (P＜0.01).In experimental group the phosphorylation of EphA2 protein and activation of MMP2 protein were much supressed by alphastatin and there was no change in VEGF protein.Conclusion Alphastatin can supress not only endothelial cell dependent blood vessel but also vasculogenic mimicry.It may be a ideal vasoinhibitory peptide.关键词
血管生成拟态/Alphastatin多肽/增殖/迁移/血管形成Key words
vasculogenic mimicry/ alphastatin / proliferation/ migration / tubule formation引用本文复制引用
郭世文,张熙,尉春艳..Alphastatin多肽对胶质瘤血管生成拟态的抑制作用及其相关机制[J].重庆医学,2011,40(21):2084-2086,3.基金项目
国家自然科学基金资助项目(30672126). （30672126）
