重庆医学2011，Vol.40Issue(21)：2094-2095,2098,3.DOI:10.3969/j.issn.1671-8348.2011.21.005
碳青霉烯耐药鲍曼不动杆菌耐药性分析及金属β-内酰胺酶检测
Detection of Metallo-β-lactamases produced by carbapenem-resistant Acinetobacter baumannii
汤荣睿 1龚雅利 2张晓兵1
作者信息
- 1. 第三军医大学西南医院检验科,重庆,400038
- 2. 重庆市沙坪坝区人民医院检验科,400030
- 折叠
摘要
Abstract
Objective To investigate antimicrobial resistance and detection of Metallo-β-Iactamases produced by carbapenem-resistant Acinetobacter baumannii.Methods Antimicrobial susceptibility test was done on 51 strains of Acinetobacter baurnannii isolated by Kirby-Bauer method.Results were assessed according to the standands recommended by CLSI (2008).Metallo-β-lactamases was detected by EDTA inhibition test.The genotype of IMP-1,IMP-2 , VIM, VIM-2 was performed by polymerase chain reaction (PCR).Results 51 strains Acinetobacter baumannii were multi-drug resistant.Sensitive to antibiotics except Amikacin were low.Positive rate of strains of IMP-1 , VIM-2 was 25.5％ , 19.6％ , respectively.But no IMP-2 , VIM was detected.Conclusion Production of Metallo-β-lactamases in Acinetobacter baumannii is one of the main mechanisms of carbapenems resistance at our hospital.It brings concern that carbapenem-resistant clone was multipleresistant strains.关键词
鲍曼不动杆菌/碳青霉烯类/耐药性/β-内酰胺酶Key words
acinetobacter baumannii / carbapenems / resistance / β-lactamases引用本文复制引用
汤荣睿,龚雅利,张晓兵..碳青霉烯耐药鲍曼不动杆菌耐药性分析及金属β-内酰胺酶检测[J].重庆医学,2011,40(21):2094-2095,2098,3.
