国际口腔医学杂志2011，Vol.38Issue(3)：268-273,6.DOI:10.3969/j.issn.1673-5749.2011.03.006
银杏酚酸提高Tca8113对化学治疗药物敏感性的研究
Ginkgolic acids can enhance the drug-sensitivity of Tca8113
戴丽娜 1苏勤 1周陈晨 1王辉 1陈娇 1张平1
作者信息
- 1. 口腔疾病研究国家重点实验室,四川大学,成都,610041
- 折叠
摘要
Abstract
Objective To investigate the effect and mechanism of ginkgolic acid (GA) on drug-sensitivity of Tca8113 cells to chemotherapy drug. Methods Methyl thiazolyl tetrazolium(MTT) assay for detection of single use of Carboplatin(CBP) or Paclitaxel(PTX) and combination of CBP or PTX with GA on Tea8ll3 cell survival rate. Western blot detection of protein expression of p-protein kinase D(PkD)-2 and PkD-2 in Tea8ll3 cell indueed by GA with different times, shRNA was introduced to downregulate the expression of PkD-2 in Tea8ll3 cell line. The proliferation activity and programmed cell death rate of shRNA mediated Tca8ll3 cells were detected by MTT and flow cytometry respectively. Results The drug-sensitivity of Tca8ll3 cells was enhanced by GA significantly; the expression of p-PkD-2 protein in Tca8113 ceils was inhibited by GA in a time-dependent manner vs PkD-2, compared to the control, shRNA targeting of PkD-2 leads to the efficient and specific silence of pkD-2 mRNA and protein expression as determined by RT-PCR and Western blot, a dramatic reduction of cell proliferation and an increased programmed cell death rate of Tea8113 cells treated with chemotherapy drug in vitro. Conclusion GA can induce Tea8ll3 cells programmed cell death by inhibiting the phosphorylation of pPkD-2, thus enhance the sensitivity of Tca8113 cells to chemotherapy drug.关键词
银杏酚酸/Tca8113/细胞增殖/程序性细胞死亡/蛋白质激酶DKey words
ginkgolic acid/ Tca8ll3/ cell proliferation/ programmed cell death/ protein kinase D分类
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戴丽娜,苏勤,周陈晨,王辉,陈娇,张平..银杏酚酸提高Tca8113对化学治疗药物敏感性的研究[J].国际口腔医学杂志,2011,38(3):268-273,6.
