安徽农业科学2011，Vol.39Issue(8)：4436-4437,4449,3.
根癌农杆菌介导烟草K326遗传转化体系优化
Optimization of Agrobacterium-mediated K326 Genetic Transformation System
赵勤1
作者信息
- 1. 四川农业大学农学院,四川雅安,625014
- 折叠
摘要
Abstract
[ Objective ] The aim was to optimize tobacco K326 genetic transformation system mediated by Agrobacterium. [ Method ] The leaf of tobacco aseptic seedling was taken as explants to study the optimization of K326 genetic transformation system via Agrobacterium. [ Result]The highest transformation efficiency was obtained when the explants was pre-cultured in the medium of MS + 2 mg/L 6-BA + 0.2 mg/L IAA for 2 d, and then infected with Agrobacterium GV3101 (OD600 = 0.6) for 5 min. The PCR detection proved that NPTII gene had been integrated into the regenerated tobacco plants. [ Conclusion ] A highly efficient genetic transformation system of tobacco leaf mediated by Agrobacterium was established.关键词
根癌农杆菌/烟草/遗传转化Key words
Agrobacterium tumefaciens/ Tobacco/ Genetic transformation分类
农业科技引用本文复制引用
赵勤..根癌农杆菌介导烟草K326遗传转化体系优化[J].安徽农业科学,2011,39(8):4436-4437,4449,3.
