安徽农业科学2011，Vol.39Issue(8)：4438-4439,4442,3.
薯蓣皂苷糖苷酶基因的克隆及同源性分析
Cloning and Homology Analysis of Dioscin-glycosidase Gene from Absidia sp. G3d
摘要
Abstract
[ Objective ] A dioscin-glycosidase from Absidia sp. G3d was studied in molecular biology. Dioscin-glycosidase gene of CDS was cloned and analyzed. [ Method ] The primers were designed according to N-terminal sequence of dioscin-glycosidase. The partial eDNA of dioscin-glycosidase gene was obtained through the method of RT-PCR using total RNA as template. Moreover, a 1 497 bp full length CDS sequence of dioscin-glycosidase was obtained using RT-PCR, 5′- RACE and 3′- RACE technique. Sequence homology analysis of dioscin-glycosidase gene was performed by BLAST and subsequently compared with GenBank data. [ Result ] There was no identity between dioscin-glycosidase gene and the known α-rhamnosidase gene in the Genbank. But there was a high homogeneity with alpha-amylase. [ Conclusion ] There was difference between the protein structures. And the reaction characteristics of the protein were different.关键词
薯蓣皂苷糖苷酶/RT-PCR/序列分析Key words
Dioscin-glycosidase/ RT-PCR / Sequence analysis分类
农业科技引用本文复制引用
吴宁,金凤燮,鱼红闪..薯蓣皂苷糖苷酶基因的克隆及同源性分析[J].安徽农业科学,2011,39(8):4438-4439,4442,3.基金项目
国家自然科学基金项目(30371744,30470055,20476017) （30371744,30470055,20476017）
辽宁省科学技术基金项目(20042132) （20042132）
辽宁省教育厅创新团队项目(2009T007). （2009T007）
