安徽医科大学学报2011，Vol.46Issue(7)：640-645,6.
介导大鼠海马CA1区癫痫样活动的离子型谷氨酸受体的变化
Variations of ionotropic glutamate receptors mediating epileptiform activity in rat hippocampal CA1 region
摘要
Abstract
Objective To investigate the variations of ionotropic glutamate receptors mediating epileptiform activity in rat hippocampal CAI region in order to explore the mechanism of epileptogenesis. Methods The epileptiform activity was induced by perfusing normal hippocampal slices with GABAA receptor antagonist bicuculline ( 30 μmol/L ) and temporal lobe epilepsy ( TLE ) model was established by kainic acid( KA ) injected into the CA3 region of the right hippocampus. The population spike ( PS ) of CAI pyramidal cells was recorded with extracellular recording in hippocampal slices by electrical stimulating Schaffer collateral in stratum radiatum. Results Single PS , which mainly mediated by non-NMDA receptors, was recorded in normal hippocampal slices. The multiple epileptiform discharges, which partially mediated by NMDA receptor in addition to non-NMDA receptors, could be induced by perfusing bicuculline. After perfusing NMDA receptor antagonist, APV ( 50 μmol/L ), the amplitude of the first PS was not significantly affected , however , the amplitude offourth and fifth PS was decreased obviouslv( n = 11 , P ＜ 0 . 0 5 ;n = 9 ,P < 0. 05 ). After perfusing APV in slices of TLE model rat. the amplitude of the first PS was not significantly affected, but the amplitude of the fourth and fifth PS was decreased obviously ( n = 8,P ＜0. 05 ; n = 8 ,P ＜ 0. 01 ).and APV could inhibit the residual potential after perfusing non-NMDA receptor antagonist, CNQX ( 10 μmol/L ).Conclusion The activation of NMDA receptor contributes to epileptiform activity in hippocampal CAI region.关键词
癫痫/海马/受体,谷氨酸Key words
epilepsy/hippocampus/receptors,glutamate分类
医药卫生引用本文复制引用
王斌生,尤竹燕,解敏,李珍,王烈成,孔德虎..介导大鼠海马CA1区癫痫样活动的离子型谷氨酸受体的变化[J].安徽医科大学学报,2011,46(7):640-645,6.基金项目
安徽省教育厅自然科学重点基金(编号:KJ2007A028) （编号:KJ2007A028）
教育部新世纪优秀人才支持计划(编号:NCET-06-0557) （编号:NCET-06-0557）
安徽省优秀青年科技基金(编号:06043090) （编号:06043090）
