中国病理生理杂志2011，Vol.27Issue(7)：1352-1359,8.DOI:10.3969/j.issn.1000-4718.2011.07.019
非酒精性脂肪性肝病在代谢综合征相关的2型糖尿病发病中的作用
Effect of non-alcoholic fatty liver disease on metabolic syndrome-rela ted type 2 diabetes
摘要
Abstract
AIM: To investigate the effect of non - alcoholic fatty liver disease ( NAFLD ) on metabolic syndrome ( MS ) - related type 2 diabetes.METHODS: Sixty - four rats were randomly divided into 8 groups: normal control groups of 3 months ( 3NC ), 6 months ( 6NC ), 9 months ( 9NC ) and 12 months ( 12NC ), and high - sucrose and high -fat groups of 3 months ( 3H ), 6 months ( 6H ), 9 months ( 9H ) and 12 months ( 12H ).The serum levels of alanine transaminase ( ALT ), free fatty acid ( FFA ), endotoxin ( LPS ), tumor necrosis factor alpha ( TNFα ), C - reactive protein ( CRP ), monocyte chemotactic protein - 1 ( MCP - 1 ), fasting plasma glucose ( FPG ) and fasting plasma insulin ( FINS ) were measured.The insulin resistance index ( HOMA - IR ) and beta cell function index( HOMA - β ) were also calculated.The specimens of liver and pancreas were prepared for microscopic observation with HE and Sudan Ⅳ staining.The visceral adipose specimens were also prepared with HE staining.The apoptosis of islet cells was detected by TUNEL.RESULTS: Compared with normal control groups, the levels of ALT, FFA, LPS, TNFα, CRP, MCP - 1, FPG, FINS and HOMA - IR in high - sucrose and high - fat groups of every phase were higher, but the level of HOMA - β in 6H group showed a compensatory increase first and then progressively decreased.Compared with normal control groups, TUNEL results showed that the number of apoptotic islet cells in high - sucrose and high - fat groups gradually increased from the 3rd to the 12th month.CONCLUSION: The NAFLD plays a trigger role in the start of MS -related type 2 diabetes.关键词
非酒精性脂肪性肝病/代谢综合征/糖尿病,2型/胰岛素抵抗Key words
Non - alcoholic fatty liver disease/ Metabolic syndrome/ Diabetes mellitus,type 2/ Insulin resistance分类
医药卫生引用本文复制引用
周鑫,韩德五,李素红,郭建红,许瑞龄,吴惠文,赵元昌..非酒精性脂肪性肝病在代谢综合征相关的2型糖尿病发病中的作用[J].中国病理生理杂志,2011,27(7):1352-1359,8.基金项目
山西省自然科学基金资助项目(No.2008011073-1) （No.2008011073-1）
