中国病理生理杂志2011，Vol.27Issue(7)：1406-1408,3.DOI:10.3969/j.issn.1000-4718.2011.07.029
尾加压素Ⅱ及其受体在急性肾损伤大鼠肾组织中的表达
Expression of urotensin II and its receptor in nephridial tissues of rats with acute renal damage
摘要
Abstract
AIM: To observe the mRNA expression of urotensin Ⅱ ( UⅡ ) and its receptor ( G protein - coupled receptor 14, GPR14 ) in nephridial tissues of rats with acute renal damage.METHODS: Male Wistar rats were divided into 2 groups: the rats in control group ( n = 10 ) were administered with normal saline by gavage; the rats in model group ( n = 30 ) were administered with Caulis Aristolochiae manshuriensis ( CAM ) by gavage for 25 d to induce acute renal damage.Every 5 rats in model group were sacrificed on the 3rd, 7th, 15th and 25th days during CAM treatment and all rats in the 2 groups were killed 10 d after withdraw of CAM.The kidneys were collected for pathological observation and the UⅡ and GPR14 mRNA examination.RESULTS: The degeneration, necrosis and disintegration in tubules were observed as major pathological changes in the rat kidneys after 3 d of CAM administration.The pathological changes were aggravated following the duration of in CAM administration, and were remained and even worsen when CAM was withdrawn for 10 d.Compared with control group, the mRNA expression of UⅡ was significantly elevated ( P ＜0.05 ) at the time point of CAM administration for 15 d,even obviously increased (P＜0.01)at the time point of CAM administration for 25 d, and remained at the highest levels to the end of the observation.The mRNA expression of GPR14 was significantly increased ( P ＜0.05 ) at the time point of CAM administration for 7 d, became higher ( P ＜0.01 ) on the 15th day, and gradually increased as the experimental time went on.CONCLUSION: The mRNA levels of UⅡ and its receptor are significantly elevated in CAM -induced renal lesion in rats, suggesting that UⅡ plays a pathological role in the development of acute renal damage.关键词
尾加压素Ⅱ/急性肾损伤Key words
Urotensin Ⅱ/ Acute renal damage分类
医药卫生引用本文复制引用
陈素贤,李才,于晓艳,石艳..尾加压素Ⅱ及其受体在急性肾损伤大鼠肾组织中的表达[J].中国病理生理杂志,2011,27(7):1406-1408,3.基金项目
国家自然科学基金资助项目(No.3057085) （No.3057085）
