中国病理生理杂志2011，Vol.27Issue(7)：1414-1418,5.DOI:10.3969/j.issn.1000-4718.2011.07.031
ADSC源性神经干细胞向神经元和星形胶质细胞不同方向分化时的超微结构变化研究
Ultrastructural characteristics of neural stem cells derived from adipose-derived stromal cells during their differentiation into neurons and astrocytes
欧亚 1元小冬 1蔡亚楠 1陆艳卉1
作者信息
- 1. 河北联合大学附属开滦医院神经内科,河北,唐山,063000
- 折叠
摘要
Abstract
AIM: To observe the ultrastructural characteristics of neural stem cells derived from adipose - derived stromal cells ( ADSC ) differentiating into neurons and astrocytes.METHODS: ADSC were cultured, amplified and induced with β - mercaptoethanol or 3 - isobutyl - 1 - methylxanthine ( IBMX ).The expression of nestin in the induced cells was determined by the method of immunofluorescence.The ultrastructure of the induced neural stem cells was observed under transmission electron microscope.RESULTS: The expression of nestin reached the peak at 3 h in β - mercaptoethanol group and at 14 d in IBMX group, and the positive rate of the former ( 86％ ) was significantly higher than that of the latter (23％ ).However, the duration of the induction in IBMX group was obviously longer than that in β - mercaptoethanol group.The ultrastructure of the induced neural stem cells in the two groups was similar under the transmission electron microscope, only with some differences in organelles.CONCLUSION: In the process of ADSC differentiating into astrocytes or neurons with the induction of β - mercaptoethanol or IBMX, the different changes of ultrastructure in ADSC -derived neural stem cells occur in some organelles in cytoplasm, not in nucleus.关键词
脂肪基质细胞/神经干细胞/神经元/星形细胞Key words
Adipose stromal cells/ Neural stem cells/ Neurons/ Astrocytes分类
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欧亚,元小冬,蔡亚楠,陆艳卉..ADSC源性神经干细胞向神经元和星形胶质细胞不同方向分化时的超微结构变化研究[J].中国病理生理杂志,2011,27(7):1414-1418,5.
