中国病理生理杂志2011，Vol.27Issue(7)：1424-1427,4.DOI:10.3969/j.issn.1000-4718.2011.07.033
二氢杨梅素联合丝裂霉素对胃癌细胞的生长抑制作用
Inhibitory effect of ampelopsin combined with mitomycin on proliferation of gastric carcinoma cells
吴明彩 1蒋明 2毕富勇1
作者信息
- 1. 皖南医学院生化教研室,安徽,芜湖241001
- 2. 二炮芜湖鸠江干休所卫生所,安徽,芜湖,241000
- 折叠
摘要
Abstract
AIM: To investigate the synergistic effects of ampelopsin ( AMP ) and a chemotherapeutic drug mitomycin ( MMC ) on the proliferation of gastric cancer cell line SGC -7901.METHODS: SGC -7901 cells were cultured in vitro and divided into 4 groups: control group, AMP group, MMC group and AMP + MMC group.Cell proliferation was measured by MTT method.The apoptotic index was examined by flow cytometry.The expression of apoptotic proteins, Bcl- 2 and survivin, was detected by Western blotting.RESULTS: AMP at the concentrations ranging from 2.2 mg/L to 14.84 mg/L exerted inhibitory effect on the growth of SGC - 7901 cells.Cell proliferation in AMP ( 14.84 mg/L ) group was inhibitory by ( 60.85 ± 1.13 ) ％ , significantly higher than that in control group ( P ＜0.05 ).The inhibitory rates of cell proliferation varied from ( 17.40 ±0.30 ) ％ to ( 72.23 ± 1.36 ) ％ when the concentrations of MMC increased from 1× 10-3 g/L to 1 × 10-2 g/L.AMP combined with MMC showed a synergistic effect on the growth of SGC -7901 cells.The inhibitory rates of cell proliferation varied from ( 21.83 ± 2.50 ) ％ to ( 46.70 ± 1.45 ) ％ when the concentrations of MMC increased from 1 × 10 -3g/L to 5 × 10-3 g/L.The inhibitory effect of AMP plus MMC was higher than that of MMC or AMP alone.The protein levels of Bcl - 2 and survivin were inhibited in AMP group, MMC group and AMP + MMC group, and were significantly lower in AMP + MMC group than those in AMP group or MMC group.CONCLUSION: AMP enhances the inhibitory effect of MMC on the growth of SGC -7901 cells.The mechanism is related to the inhibition of apoptotic protein expression.关键词
二氢杨梅素/丝裂霉素/胃肿瘤/SGC-7901细胞/细胞凋亡Key words
Ampelopsin/ Mitomycin/ Stomach neoplasms/ SGC-7901 cells/ Apoptosis分类
医药卫生引用本文复制引用
吴明彩,蒋明,毕富勇..二氢杨梅素联合丝裂霉素对胃癌细胞的生长抑制作用[J].中国病理生理杂志,2011,27(7):1424-1427,4.
