中国病理生理杂志2011，Vol.27Issue(7)：1450-1452,1456,4.DOI:10.3969/j.issn.1000-4718.2011.07.038
线栓法复制昆明小鼠局灶性脑缺血再灌注模型的稳定性研究
Stability of cerebral focal ischemia/reperfusion model induced by monofilament in Kunming mice
摘要
Abstract
AIM: To study the stability of mouse cerebral ischemia/reperfusion model induced by the method of monofilament.METHODS: Sixty male Kunming mice were divided into 3 groups according to the body weight: group A ( 18 - 21 g ), group B ( 22 - 28 g ) and group C ( 30 - 35 g ).Ischemia/reperfusion ( I/R ) model was made by middle cerebral artery occlusion ( MCAO ) with nylon monofilament.To evaluate the mouse MCAO model, the method of PRM2 laser Doppler was used to detect the cerebral blood flow, the neurological deficit scores were determined by Longa standard and infarction volume was detected with TTC staining.The content of malondialdehyde ( MDA ) and activity of superoxide dismutase ( SOD ) were also measured by ELISA.RESULTS: The successful rates of model establishment in both group A and group B were higher than that in group C ( P ＜ 0.05 ), especially the highest in group B.The mortality in group A was significantly higher than that in group B and group C ( P ＜ 0.05 ).The behavior scores and cerebral infarct volume in group A and group B were significantly higher than those in group C ( P ＜ 0.05 ).Obvious brain injury and neurological deficits were also observed in group A and group B with the higher content of MDA and the lower activity of SOD in the cerebral cortex of the injury side.CONCLUSION: There are three important factors to ensure the success and stability of MCAO mouse model induced by monofilament, i.e.the diameter of monofilament matching the body weight of the mice, the suitable length of monofilament within the blood vessel, as well as the maintaining of proper room temperature during experiment.The MDA content and SOD activity are also effective indexes for evaluating the cerebral I/R injury.关键词
小鼠/脑缺血再灌注/脑血流/脂质过氧化作用Key words
Mice/ Brain ischemia/reperfusion/ Brain blood flow/ Lipid peroxidation分类
医药卫生引用本文复制引用
赵瑾,苑琳,王驭龙,潘慧,祝世功..线栓法复制昆明小鼠局灶性脑缺血再灌注模型的稳定性研究[J].中国病理生理杂志,2011,27(7):1450-1452,1456,4.基金项目
国家自然科学基金资助项目(No.30770804 （）
No.30871034 ) （）
安徽省教育厅基金资助项目(No.KJ2010B122) （No.KJ2010B122）
国家基础科学人才培养基金资助项目(No.J0630853 /J0108) （No.J0630853 /J0108）
