中国组织工程研究与临床康复2011，Vol.15Issue(19)：3507-3512,6.DOI:10.3969/j.issn.1673-8225.2011.19.020
高压氧联合神经干细胞移植治疗大鼠脊髓损伤
Hyperbaric oxygen combined with neural stem cells transplantation for rat spinal cord injury
石瑞成 1冯士军 1王志刚1
作者信息
- 1. 包头医学院第一附属医院神经外科,内蒙古自治区包头市,014010
- 折叠
摘要
Abstract
BACKGROUND: Neural stem cells (NSCs) transplantation is widely used in the repair of spinal cord injury (SCI).OBJECTIVE: To investigate the effect of NSCs transplantation plus hyperbaric oxygen (HBO) on repair of rat SCI.METHODS: Sixty female rats were divided randomly into three groups: SCI group; NSCs transplantation group; NSCs transplantation+HBO group. Four weeks post-transplantation, histopathology examination and BrdU staining were performed.Eight weeks post-transplantation, horseradish peroxidase (HRP) nerve trace and transmission electron microscope testing and somatosensory evoked potential (SEP) testing were performed. At 1 , 2, 4, 6, 8 weeks post-injury, all animals were evaluated for the hind limb behavior with Basso-Beattle-Bresnahan (BBB) score and inclined plane test.RESULTS AND CONCLUSION: HRP-Iabeled neurofibra and BrdU positive cells were found in the spinal cord of the NSCs transplantation+HBO group, some in NSCs transplantation group but few in the SCI group spinal cord injury. SEP testing in the SCI group surpassed that in the NSCs transplantation group (P ＜ 0.05). There was significant difference between spinal cord injury group and NSCs transplantation+ HBO group (P ＜ 0.01). By transmission electron microscope, nerve fibers and myelinated nerve fibers were found in the central transverse plane in the NSCs transplantation group and NSCs transplantation+HBO group.The outcome of functional recovery in the NSCs transplantation+HBO group surpassed that in the NSCs transplantation group.There was significant difference between the NSCs transplantation and NSCs transplantation+ HBO groups (P ＜ 0.05). The outcome of the SCI group was worse than that of the other groups. There was significant difference between the NSCs transplantation group, NSCs transplantation+ HBO group and SCI group (P ＜ 0.05). NSCs transplantation can promote the functional recovery of rat hind limbs after SCI, and its combination with HBO has a synergistic effect.关键词
脊髓损伤/神经干细胞/移植/高压氧/运动功能分类
基础医学引用本文复制引用
石瑞成,冯士军,王志刚..高压氧联合神经干细胞移植治疗大鼠脊髓损伤[J].中国组织工程研究与临床康复,2011,15(19):3507-3512,6.
