中国组织工程研究与临床康复2011，Vol.15Issue(19)：3525-3527,3.DOI:10.3969/j.issn.1673-8225.2011.19.024
超低温冰箱转液氮阶梯降温法与传统程序降温法保存造血干细胞的比较
Cryopreservation of hematopoietic stem cells with ladder-style freezing from low temperature refrigerator to liquid nitrogen method versus programmed cooling method
摘要
Abstract
BACKGROUND:During the cryopreservation of hematopoietic stem cells, there are many influential factors, such as cooling rate, storage temperature, and cryoprotectant combination. There is a controversy in the cryopreservation methods. OBJECTIVE: To investigate the differences between -80 ℃ ladder-style freezing from low temperature refrigerator to liquid nitrogen method and programmed cooling method in preserving peripheral blood stem cells (PBSCs). METHODS: Collected PBSCs were divided into two groups, and respectively cryopreserved by using -80 ℃ ladder-style freezing from low temperature refrigerator to liquid nitrogen method and programmed cooling method. RESULTS AND CONCLUSION: There was no significant difference between these two frozen samples of PBSCs after recovery in the aspects of trypan blue exclusion rate, recovery, apoptosis rate and mortality (P ＞ 0.05). These findings indicate that the -80 ℃ ladder-style freezing from low temperature refrigerator to liquid nitrogen method using the cryoprotectant containing 5％ dimethyl sulfoxide, 4％ albumin and 3％ hydroxyethyl starch can yield the same effect as same as the programmed cooling method using 10％ DMSO as cryoprotectant in the aspect of PBSCs cryopreservation, and the former one is more convenient and more suitable for clinical application.关键词
冷冻保护剂/程序降温/造血干细胞/非程序降温/二甲基亚砜分类
医药卫生引用本文复制引用
赵仁彬,张爱玲,陆洁,杨泽凤,杨同华..超低温冰箱转液氮阶梯降温法与传统程序降温法保存造血干细胞的比较[J].中国组织工程研究与临床康复,2011,15(19):3525-3527,3.基金项目
云南省科技厅-昆明医学院联合专项基金资助项目(2007C00036R). （2007C00036R）
