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魟鱼软骨多糖的聚丙烯酰胺凝胶电泳鉴别

王洪建 郭斌

安徽农业科学2011,Vol.39Issue(8):4734-4735,2.
安徽农业科学2011,Vol.39Issue(8):4734-4735,2.

魟鱼软骨多糖的聚丙烯酰胺凝胶电泳鉴别

Identification of Ray Cartilage Glycosaminoglycans by Polyacrylamide Gel Electrophoresis

王洪建 1郭斌2

作者信息

  • 1. 辽宁医学院,辽宁锦州,121001
  • 2. 辽宁医学院附属一院,辽宁锦州,121001
  • 折叠

摘要

Abstract

[ Objective ] This paper aims to study the feasibility of qualitative determination of Ray Cartilage Glycosaminoglycans (RCG) by polyacrylamide gel electrophoresis. [ Methods ] Pure RCG is applied to conduct phosphate buffer of polyacrylamide gel electrophoresis. [ Results ] After being purified, RCG realizes seclusion of amylase. According to the electrophoretic mobility of polyacrylamide gel electrophoresis,RCG could be obviously identified. [ Conclusions] This method has good repeatability and could be used for the qualitative identification of RCG.

关键词

虹鱼软骨多糖/纯化/聚丙烯酰胺凝胶电泳/鉴别

Key words

Ray Cartilage Glycosaminoglycans/ Purification/ Polyacrylamide gel electrophoresis/ Identification

分类

农业科技

引用本文复制引用

王洪建,郭斌..魟鱼软骨多糖的聚丙烯酰胺凝胶电泳鉴别[J].安徽农业科学,2011,39(8):4734-4735,2.

基金项目

辽宁省教育厅基金项目(202173371). (202173371)

安徽农业科学

0517-6611

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