中国组织工程研究与临床康复2011，Vol.15Issue(19)：3567-3570,4.DOI:10.3969/j.issn.1673-8225.2011.19.035
葡萄糖浓度与大鼠胰腺导管干细胞的诱导分化
Effects of glucose concentration on differentiation of rat pancreatic duct stem cells
陈昊强 1史光军 2许评 2吴晓平3
作者信息
- 1. 青岛大学医学院,山东省青岛市,266071
- 2. 青岛市市立医院东院肝胆外科,山东省青岛市,266071
- 3. 青岛市市立医院细胞及肝胆胰实验室,山东省青岛市,266071
- 折叠
摘要
Abstract
BACKGROUND: Glucose is an important factor on differentiation of pancreatic duct stem cells, it relates to the quantity and secretion function of insulin-producing cells after differentiation.OBJECTIVE: To compare the insulin secretion capacity of the differentiated rat pancreatic stem cells induced by various glucose concentrations.METHODS: Rat stem cells were isolated and purified from pancreatic duct cells using collagenase V and Ficoll-400. These stem cells were randomly divided into 10 groups. Every group was induced to culture, proliferate, differentiate and form insulin- producing cells in vitro. The differentiation of all groups was performed in medium with different concentrations of glucose. The immunofluorescence staining was used to identify the pancreatic duct stem cells. The electrochemical luminescence method was used to detect the insulin release from stem cell differentiated islets.RESULTS AND CONCLUSION: The stimulation index of glucose 20.6, 25.6, 30.6 mmol/L groups was higher than that in other groups (P < 0.05), but there was no difference between each two groups among these three groups (P > 0.05). The insulin releasing of glucose 15.6, 20.6, 25.6 groups was higher than that in other groups (P < 0.05), but there was no difference between each two groups among these three groups (P > 0.05). The best insulin secretion capacity of insulin-producing cells can be gained by controlling concentration of glucose as 20.6-25.6 mmol/L when pancreatic duct stem cells differentiated into insulin-producing cells.关键词
胰岛移植/胰腺导管干细胞/葡萄糖浓度/胰岛素/分泌量分类
医药卫生引用本文复制引用
陈昊强,史光军,许评,吴晓平..葡萄糖浓度与大鼠胰腺导管干细胞的诱导分化[J].中国组织工程研究与临床康复,2011,15(19):3567-3570,4.
