中国人兽共患病学报2011，Vol.27Issue(5)：367-370,4.
登革3型病毒义乌流行株免疫原性研究
Biological characteristics of dengue virus type 3 isolated from Yiwu,China
摘要
Abstract
To study the biological characteristics of dengue virus type 3 isolated from Yiwu, China, the genome was amplified by RT-PCR, and phylogenetic trees were constructed by MEGA 4 based on E and NS1 nucleotide and amino acid.E protein expression plasmid named JESS-ywD3prM/E397JEV -pcDNA5 was constructed and transfected into 293T cells, the supernatants containing secreted E protein were purified by sucrose density gradient centrifugation and inoculated into BALB/c mice,the humoral immunity was evaluated by ELISA and neutralization analysis.Phylogenetic tree analysis showed that the similarity with Guangzhou strain GZ1D3 and India strain GWL-25 were reached above 99％.Secreted E protein could induce dengue specific IgG antibodies and neutralizing antibodies against homotypic dengue virus.关键词
登革3型病毒/义乌分离株/生物学特性Key words
Dengue virus type 3/ strain isolated from Yiwu/ biological characteristics分类
医药卫生引用本文复制引用
顾雯,李德新,金聪,张硕,张全福,李建东,杭小同,王芹,李川,梁米芳..登革3型病毒义乌流行株免疫原性研究[J].中国人兽共患病学报,2011,27(5):367-370,4.基金项目
国家高技术研究发展计划项目(2006AA02A223)资助 （2006AA02A223）
