中国组织工程研究与临床康复2011，Vol.15Issue(21)：3851-3854,4.DOI:10.3969/j.issn.1673-8225.2011.21.012
改良自乳化溶剂扩散法制备MePEG-PLA纳米粒对成骨细胞的毒性
Cytotoxicity effect of MePEG-PLA nanoparticles prepared by modified self-emulsion solvent diffusion technique on osteoblasts in vitro
摘要
Abstract
BACKGROUND: Amphiphilic block copolymers have been widely applied in the drug delivery system because of the high capacity of drug carriers, nanoparticles and long circulation time. OBJECTIVE: To evaluate the cytotoxicity effect of MePEG-PLA nanoparticles prepared by modified self-emulsion solvent diffusion technique on MG63 in vitro.METHODS: MePEG-PLA nanoparticles were prepared by modified self-emulsion solvent diffusion technique. The cytotoxicity effect of MePEG-PLA nanoparticles on MG63 was detected using MTS assay at days 1, 2, 3. Particles size, polydispersity index and Zeta electric potential were measured by laser particle size analzer. The morphology of MePEG-PLA nanoparticles were observed by transmission electron microscope. Absorbance values at days 1, 2, 3 were detected by microplate reader. RESULTS AND CONCLUSION: MePEG-PLA nanoparticles had a regular spherical shape with the mean diameter of 25.7 nm and scattered evenly. The Zeta potential was -8.06 mV. The cytotoxicity grade of MePEG-PLA nanoparticles was 0. The results indicate that MePEG-PLA nanoparticles prepared easily by modified self-emulsion solvent diffusion technique are nontoxic to the MG63 and have a good prospect.关键词
纳米粒/药物载体/聚乳酸/聚乙二醇/改良自乳化溶剂扩散法分类
医药卫生引用本文复制引用
王忠磊,高岩,赖春花,卢海宾,李少冰,蒋颖,韦丛云,周磊..改良自乳化溶剂扩散法制备MePEG-PLA纳米粒对成骨细胞的毒性[J].中国组织工程研究与临床康复,2011,15(21):3851-3854,4.基金项目
the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period,No.2007BA I18BO6 （）
