中国组织工程研究与临床康复2011，Vol.15Issue(21)：3871-3876,6.DOI:10.3969/j.issn.1673-8225.2011.21.017
可塑性纳米羟基磷灰石/聚羟基丁酸酯-羟基戊酸酯共聚物-聚乙二醇释药系统治疗兔骨髓炎
Treatment of osteomyelitis in rabbits with plastic nanohydroxyapatite/poly (3-hydroxybutyrate-hydroxyvalerate)-polyethylene glycol drug delivery system
章柏平 1汤善华 1张立 1吕仁发 1卢海峰 1靳安民 2王旭东3
作者信息
- 1. 解放军第184医院骨科,江西省鹰潭市335000
- 2. 南方医科大学珠江医院骨科,广东省广州市510282
- 3. 华南理工大学生物工程与材料学院,广东省广州市510200
- 折叠
摘要
Abstract
BACKGROUND: Existing local drug delivery system is mostly massive, cannot arbitrary moulding and is difficult to completely sticking with the bone cavity, which easily lead to recurrence of osteomyelitis, and obvious effect of drug burst release, small amount of drug loading, short releasing time, and certain antigenicity. Therefore, a new type of plastic local drug delivery system was developed by task force and South China University of Technology, in order to provide a better method of treatment of osteomyelitis.OBJECTIVE: To investigate the therapeutic effect of the plastic nanohydroxyapatite/poly(3-hydroxybutyrate-hydroxyvalerate)-polyethylene glycol-gentamicin local drug delivery system (nano-HA/PHBV-PEG-GM-DDS) on osteomyelitis.METHODS: Proximal tibia osteomyelitis model of rabbits was prepared. Staphylococcus aureus was injected into proximal tibia bone window after 2 weeks and underwent debridement. The plastic nano-HA/PHBV-PEG-GM-DDS of 1 mL was implanted into groupⅠ, the plastic nano- HA/PHBV-PEG of 1 mL mixed gentamicin power of 23.2 mg into group Ⅱ, the plastic nanoHA/PHBV-PEG of 1 mL in conjunction with intramuscle gentamicin into group Ⅲ 5 days, a total of 23.2 mg, the plastic nano-HA/PHBV-PEG of 1 mL without antibiotic in group Ⅳ, and nothing into group Ⅴ. Specimens were harvestd 8 weeks after the above procedures and then were subjected to radiological, histological and bacteriological examinations.RESULTS AND CONCLUSION: In groupⅠ, the bacteria counting and X-ray Norden scoring were by far the smallest among all 5 groups (P ＜ 0.01)，with no histological manifestation of osteomyelitis. In groupⅡ, Ⅲ, and Ⅳ, the bacteria counting gradually increased and there was significant difference among three groups (P ＜ 0.01). However, there was no significant difference in improved X-ray Norden scoring among 3 groups (P ＞0.05); histological manifestation had also no essential difference. The bacteria counting and Norden scoring as well as the histological manifestation in groupⅡ, Ⅲ, and Ⅳ were significantly higher than those in group Ⅴ (P ＜ 0.01 or 0.05), its histology showed severe osteomyelitis manifestation. The results showed that the plastic nano- HA/PHBV-PEG-GM-DDS could be implanted as primary graft into the remaining infected defect after debridement to effectively treat osteomyelitis. Conventional systemic antibiotic or simple local antibiotic following debridement was not effective in treating chronic osteomyelitis. Primary bone grafting would rather make the condition worse, under without any antibiotic or conventional systemic antibiotic or simple local antibiotic.关键词
药物缓释系统/可塑性/纳米羟基磷灰石/骨髓炎/庆大霉素分类
医药卫生引用本文复制引用
章柏平,汤善华,张立,吕仁发,卢海峰,靳安民,王旭东..可塑性纳米羟基磷灰石/聚羟基丁酸酯-羟基戊酸酯共聚物-聚乙二醇释药系统治疗兔骨髓炎[J].中国组织工程研究与临床康复,2011,15(21):3871-3876,6.
