中国组织工程研究与临床康复2011，Vol.15Issue(19)：3607-3610,4.DOI:10.3969/j.issn.1673-8225.2011.19.045
骨髓间充质干细胞增殖与成骨分化中电磁场激活ERK通路的作用
Effects of electromagnetic field activated-ERK signaling pathway on proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND: It has been demonstrated that electromagnetic field (EMF) can adjust proliferation and differentiation of bone marrow mesenchymal stem cells, but the specific mechanism is not clear. OBJECTIVE: To investigate the effects of EMF-activated ERK1/2 pathway on proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells.METHODS: The 3rd passage of rat bone marrow mesenchymal stem cells were received EMF treatment (15 Hz, 1 mT, sine wave), 20 μmol/L PD98059 + EMF treatment, or only PD98059 treatment. Simultaneously, a normal control group was established. Western blotting was applied to detect the activation of ERK signal pathway after EMF exposure. MTT assay was used to determine the activation of proliferation of cells. And alkaline phosphatase (ALP) activity in cells was detected by an ALP kit. RESULTS AND CONCLUSION: The ERK1/2 phosphorylation, proliferation and ALP activity of rat bone marrow mesenchymal stem cells were remarkably increased after exposure to EMF (P < 0.01). PD98059 could effectively block the increasing of ERK1/2 phosphorylation and cell proliferation (P < 0.01), but elevate ALP activity in a certain level (P < 0.01). EMF stimulation can fast activate ERK1/2 signal pathway and then promote the proliferation of rat bone marrow mesenchymal stem cells, however, ERK1/2 signal pathway activation has a less effect on osteogenic differentiation of bone marrow mesenchymal stem cells.关键词
电磁场/ERK信号通路/骨髓间充质干细胞/细胞增殖/分化分类
基础医学引用本文复制引用
杨国华,吴华,赵东明..骨髓间充质干细胞增殖与成骨分化中电磁场激活ERK通路的作用[J].中国组织工程研究与临床康复,2011,15(19):3607-3610,4.基金项目
国家自然科学基金面上项目(50477043).the National Natural Science Foundation of China,No.50477043 （50477043）
