中国组织工程研究与临床康复2011，Vol.15Issue(21)：3811-3814,4.DOI:10.3969/j.issn.1673-8225.2011.21.004
壳聚糖-酪蛋白磷酸肽修饰钛合金表面的成骨细胞黏附与增殖
Adhesion and proliferation of osteoblasts on the surface of titanium alloy modified by chitosan-casein phosphopeptides
摘要
Abstract
BACKGROUND: The surface of titanium alloy only absorbs some organic molecule to activate the growth factor and stimulate cell proliferation, thereby activating the surrounding bone cells and producing a marked effect.OBJECTIVE: To prepare a bioactive coating on the surface of the titanium alloy implant, and to evaluate one promising strategy to enhance osseointegration. METHODS: The surface of titanium alloy modified by silane coupling agent was covalently grafted with chitosan (CS) and the concentration of 0, 5, 10 g/L casein phosphopeptides (CPP) in turn to form composite coatings using multi-step assembly method, compared with unmodified titanium alloy. The biocompatibility of the fabricated coatings was evaluated through co-culture of the titanium alloy and osteoblasts that modified through different procedures, using cell counting, fluorescence staining, and MTT colorimetric assay.RESULTS AND CONCLUSION: Compared with the titanium alloy without modification, the early adhesion and proliferation of surface of titanium alloy were significantly increased after CS covalently grafted with CPP (P ＜ 0.05). Moreover, with the increase of CPP concentration, the promotion enhanced significantly (P ＜ 0.05). (The osteoblasts on the surface of titanium alloy without modification displayed smooth surface, clear boundary, not fully stretched; the osteoblast cell body of the surface of titanium alloy increased significantly, rough surface, fuzzy boundaries, and fully stretched after modification by CS -CPP composite coating). It is indicated that the prepared CS-CPP composite coating can significantly increased the function of osteoblasts, improvebiological properties of titanium alloy, and expected to be an effective method to promote osseointegration.关键词
钛合金/壳聚糖/酪蛋白磷酸肽/成骨细胞/生物相容性分类
医药卫生引用本文复制引用
王琳,卜银忠,王金清,杨生荣,刘斌..壳聚糖-酪蛋白磷酸肽修饰钛合金表面的成骨细胞黏附与增殖[J].中国组织工程研究与临床康复,2011,15(21):3811-3814,4.基金项目
the National Natural Science Foundation of China,No.50801065 （）
100 Talents Program of the Chinese Academy of Sciences （）
Science and Technology Planning Project of Lanzhou Technology Bureau,No.2008-1-86 （）
Open Program of National State Key Laboratory of Solid Lubrication,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,No.0808 （）
