南方农业学报2011，Vol.42Issue(5)：539-543,5.
广西野鸟H9N2亚型流感病毒的分离与鉴定
Isolation and identification of H9N2 subtype influenza virus from wild bird in Guangxi
摘要
Abstract
[Objective]The present study was aimed to investigate the presence and spread of H9N2 subtype influenza virus in wild bird in order to understand its trends of occurrence and to develop proper control measures for preventing its infection on human beings in Guangxi. [Method]The lung tissue was collected from wild birds of Guangxi, the virus was isolated and inoculated to 9-11 day chicken-Embryo. The HA and NA subtypes were identified using serological tests followed by amplification and sequencing of HA and NA genes. [Result]The isolates were found to be inhibited by H9 subtypes positive serum, and identified as N2 subtype by neuraminidase test. This isolate belonged to the HgN2 subtype of avian influenza virus and named as A/wild bird/GuangxiHl2/07. The sequence analysis results of HA and NA genes showed 82.4-99.0％ and 83.1-99.9％ homologies of this virus strain with H9 and N2 subtypes, respectively. [Conclusion]This study proved that the H9N2 subtype influenza vires exists in wild birds of Guangxi, and provided the reference for monitoring and control of Guangxi influenza epidemic situations.关键词
野鸟/H9N2亚型流感病毒/分离/鉴定/HA基因/NA基因/广西Key words
H9N2 subtype AIV/ isolation and identification/ HA and NA genes/ Guangxi分类
农业科技引用本文复制引用
黄胜斌,蒋家霞,何奇松,付薇,孙翔翔,马琳,熊毅..广西野鸟H9N2亚型流感病毒的分离与鉴定[J].南方农业学报,2011,42(5):539-543,5.基金项目
广西科技攻关项目(桂科攻0719004-3G) （桂科攻0719004-3G）
