中国人兽共患病学报2011，Vol.27Issue(5)：390-393,4.
耐多药结核分枝杆菌对喹诺酮类药物的耐药性与gyr基因突变的初步研究
Quinolone resistance and gyr gene mutations in multi-drug resistant of Mycobacterium tuberculosis
摘要
Abstract
In order ot analyze the characteristics of gyr gene mutations in clinical isolates from the patients with multidrug resistant tuberculosis (MDR-TB) and the relation between MDR-TB with quinolone resistance and gyr gene mutations,the susceptibility of the MDR-TB clinical isolates to quinolones was tested by the proportion method.Gyr gene mutations of MDR-TB strains were detected by direct DNA sequencing.The results showed that there were 50 strains with quinolone resistance in 125 MDR-TB clinical isolates.The quinolone resistance rate was 40％.There were 40 with gyr mutations in 50 MDRTB with quinolone resistance.Of 40 quinolone resistant MDR-TB with gyr mutations, 39 mutated at condon 90, 91 and 94 of gyrA gene with a mutation rate of 78％.For 5 gyrB mutatans, 4 were associated with gyrA gene mutations.The mutation sites of gyrB were at condon 500, 506, 534 and 539 of gyrB gene.This study shows that the situation of MDR-TB with quinolone resistance is very serious.The mechanism of quinolone resistance in MDR-TB is mainly in connection with the mutation of gyrA gene.关键词
喹诺酮/gyrA基因/gyrB基因/耐多药结核分枝杆菌/耐药决定区Key words
quinolone/ GyrA gene/ GyrB gene/ multi-drug resistant Mycobacterium tuberculosis/ resistant determining regions分类
医药卫生引用本文复制引用
赵丽丽,夏强,赵秀芹,刘志广,万康林..耐多药结核分枝杆菌对喹诺酮类药物的耐药性与gyr基因突变的初步研究[J].中国人兽共患病学报,2011,27(5):390-393,4.基金项目
国家"十一五"重大传染病防治科技重大专项"结核病传播模式研究"(2008Zx100/03-010-02) （2008Zx100/03-010-02）
