中国人兽共患病学报2011，Vol.27Issue(5)：398-402,5.
猪囊尾蚴谷胱甘肽S-转移酶的表达研究
Expression of glutathione S-transferase in Taenia solium cysticercus
摘要
Abstract
Glutathione S-transferase(GST) was detected with immunohistochemistry methods, two dimensional electrophoresis and western blotting to explore the expression of GST in cysticercus cellulosae. Six piglets of 20-day-old were infected with a dose of 80 000 eggs of T.sodium and the immunohistochemistry have been done on paraffin section of cysticercus cellulosae acquired on 40, 80 and 120 days after infection from muscle and liver ( except 120 days). The total protein of cysticercus cellulosae from muscle acquired 40 days after infection was separated by two-dimensional electrophoresis, and the western blotting was performed to find out GST after the protein spots on the gel were transferred to PVDF membrane. The results showed that there was GST expressing in the head and wall of the cysticercus cellulosae from different tissues at different times. As the time passed, the GST expression of cysticercus celluosae from muscle were little changed (P＞0. 05), while the GST expression of cysticercus celluosae from brain were slightly higher(P＜0. 05). There were 207±9 protein spots of cysticercus cellulosae in 2-DE gel stained by coomassie brilliant blue, and the ranges of Mr and pI were 14 400-94 000 and 3.0-10.0, respectively. Western blotting showed 1 specific antigen spots with anti-GST mixed sera of the experimental rats and none specific spot with the mixed sera of the healthy rats. The spot in the western blotting was corresponding to the protein spot in 2-DE gel. The pI/Mr of the protein in 2-DE gel was similar to the theoretical value of GST of Taenia solium. The results indicate that there is a little difference on GST expression in cysticercus cellulosae parasited in different tissues at different times.关键词
猪带绦虫/囊尾蚴/谷胱甘肽S-转移酶/免疫组化/双向电泳/蛋白质印迹Key words
Taenia solium/ cysticercus cellulosae/ glutathione S-transferase/ immunohistochemistry/ two-dimensional electrophoresis/ western blotting分类
医药卫生引用本文复制引用
方文,肖靓靓,包怀恩,牟荣..猪囊尾蚴谷胱甘肽S-转移酶的表达研究[J].中国人兽共患病学报,2011,27(5):398-402,5.基金项目
国家自然科学基金(No.30660168)资助 （No.30660168）
