中国人兽共患病学报2011，Vol.27Issue(5)：411-413,417,4.
一株马鼻疽诺卡菌临床分离株的鉴定
Identification of Nocardia farcinica isolated from clinical strains
摘要
Abstract
To identify the species of Nocardia (N.) clinical isolates by DNA sequencing, BJ7 isolated from sputum of a patient suspected with tuberculosis, N. farcinica AY756551 and Mycobacterium tuberculosis H37Rv were used in this study.The identification on the strain was done based on culturing with L-J, PNB/TCH media and molecular biology methods. The special fragments of rpoB and 16S rDNA gene were amplified by PCR. The PCR amplification products of rpoB and 16S rDNA genes were sequenced and the DNA sequences were blasted on NCBI. The results of morphological characteristics and PNB/TCH medium showed that BJ7 was Non-tuberculosis Mycobacterium (NTM). The DNA homology of rpoB and 16S rDNA genes of BJ7 compared with that of AY756551 were 99％ and 100％ respectively. The results showed that the clinical isolate BJ7 was N. farcinica. It shows that sequencing analysis is a rapid and reliable method for Nocardia. spp species identification.关键词
非结核分枝杆菌/马鼻疽诺卡菌/菌种鉴定/测序分析Key words
Non-tuberculosis Mycobacterium/ Nocardia farcinica / species identification/ sequencing analysis分类
医药卫生引用本文复制引用
李晓亮,张媛媛,赵平,赵秀芹,陈祥,焦新安,万康林..一株马鼻疽诺卡菌临床分离株的鉴定[J].中国人兽共患病学报,2011,27(5):411-413,417,4.基金项目
国家自然科学基金项目(No.30800029)和国家"十一五"重大传染病防治科技重大专项"结核病传播模式研究"(2008ZX100/03-010-02)联合资助 （No.30800029）
(李晓亮和张媛媛在本文中具有同等贡献) （李晓亮和张媛媛在本文中具有同等贡献）
