中国人兽共患病学报2011，Vol.27Issue(5)：427-431,5.
申克孢子丝菌菌丝相和酵母相cDNA消减文库的构建及差异表达基因的筛选
Construction of cDNA subtractive library between yeast and mycelium phase of Sporothrix schenckii and screening of differently expressed genes about dimorphic transition
摘要
Abstract
The purpose of this study was to screen the differentially expressed genes about dimorphic transition of Sporothrix schenckii by construction of eDNA subtractive library between yeast and mycelium phase.The eDNA subtractive library between yeast and mycelium phase was constructed by suppression subtractive hybridization(SSH)and bioinformatics analysis was performed to profile the relationship between those differently expressed genes and dimorphic transition.Of 751 and 875 ESTs were obtained in M+Y and Y+M library, separately.After splicing of ESTs, 101 and 249 unigenes were obtained in M +Y and Y+ M libraries.During the construction of cDNA subtractive libraries, the distribution of differently expressed genes varied with dimorphic transition.The over expressed genes with diversity and complexity were divided into structural genes,metabolic enzymes, molecule on cell surface and molecule with indistinct function.Results disclose that construction of cDNA subtractive libraries for the dimorphic transition and bioinformatics analysis for those differently expressed genes lay the foundation for screening the related genes involved in the pathogenesis of Sporothrix schenckii infection.关键词
申克孢子丝菌/抑制性消减杂交/cDNA消减文库/生物信息学分析Key words
S.schenckii/ suppression subtractive hybridization(SSH)/ cDNA subtractive library/ bioinformatics analysis分类
医药卫生引用本文复制引用
周汛,杨致邦,肖异珠..申克孢子丝菌菌丝相和酵母相cDNA消减文库的构建及差异表达基因的筛选[J].中国人兽共患病学报,2011,27(5):427-431,5.基金项目
重庆市卫生局科学基金(No.2008-2-73)资助 （No.2008-2-73）
